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Resolvin E1は骨芽細胞におけるIL-17誘導性RANKL発現とRANKL誘導性破骨細胞分化を抑制することで破骨細胞形成および骨吸収を抑制する
https://repository.lib.tottori-u.ac.jp/records/5872
https://repository.lib.tottori-u.ac.jp/records/587299c7b748-7f7b-47a5-91fa-5a5e15eef4ae
| 名前 / ファイル | ライセンス | アクション |
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本文 : Full Text (2.4 MB)
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要旨 : Summary (531.0 kB)
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審査結果要旨 : Summary of Thesis Review (475.9 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2018-06-22 | |||||
| タイトル | ||||||
| タイトル | Resolvin E1は骨芽細胞におけるIL-17誘導性RANKL発現とRANKL誘導性破骨細胞分化を抑制することで破骨細胞形成および骨吸収を抑制する | |||||
| 言語 | ja | |||||
| タイトル | ||||||
| タイトル | Resolvin E1 Inhibits Osteoclastogenesis and Bone Resorption by Suppressing IL-17-induced RANKL Expression in Osteoblasts and RANKL-induced Osteoclast Differentiation | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | interleukin-17 | |||||
| キーワード | ||||||
| 主題 | osteoblasts | |||||
| キーワード | ||||||
| 主題 | osteoclasts | |||||
| キーワード | ||||||
| 主題 | RANK ligand | |||||
| キーワード | ||||||
| 主題 | resolvin E1 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | interleukin-17 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | osteoblasts | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | osteoclasts | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | RANK ligand | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | resolvin E1 | |||||
| 資源タイプ | ||||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| 著者 |
舟木, 佳弘
× 舟木, 佳弘 |
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| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 【Background】 Resolvin E1 (RvE1) derived from the ω-3 polyunsaturated fatty acid eicosapentaenoic acid is known to be a potent pro-resolving lipid mediator that prevents chronic inflammation and osteoclastogenesis. We investigated the inhibitory effects of RvE1 on osteoclastogenesis and bone resorption to clarify its therapeutic potential for rheumatoid arthritis (RA). 【Methods】 Receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation was assessed with tartrate-resistant acid phosphatase staining. RANKL-induced bone resorption was assessed by the measurement of pit formation using calcium phosphate-labeled fluorescent polyanionic molecules in RAW264.7 cells as osteoclast precursors. The effects of RvE1 on the RANKL-induced mRNA expression of osteoclast-specific genes and transcriptional factors such as c-fos and nuclear factor of activated T cells c1 (NFATc1) in RAW264.7 cells were measured by quantitative real-time PCR. The distribution of NFATc1 induced by RANKL was evaluated by immunofluorescence staining in RAW264.7 cells. To analyze the mechanism of the inhibitory effect of RvE1 on osteoclastogenesis, we measured IL-17-induced RANKL mRNA expression in MC3T3-E1 osteoblast cells treated with RvE1 using quantitative real-time PCR and determined the level of prostaglandin E2 (PGE2) production by enzyme-linked immunosorbent assay. 【Results】 RvE1 significantly suppressed RANKL-induced osteoclast differentiation and bone resorption. RvE1 inhibited the RANKL-induced mRNA expression of osteoclast-specific genes along with the transcription factors NFATc1 and c-fos. Moreover, NFATc1 translocation from the cytoplasm to the nucleus of RAW264.7 cells was suppressed following RvE1 treatment. RvE1 also inhibited IL-17-induced RANKL mRNA expression and PGE2 production in MC3T3-E1 cells. 【Conclusion】 RvE1 inhibited osteoclastogenesis and bone resorption by suppressing RANKL-induced NFATc1 and c-fos expression in osteoclasts and IL-17-induced RANKL expression through the autocrine action of PGE2 in osteoblasts. Our data suggest RvE1 as a new therapeutic target of RA. | |||||
| 学位名 | ||||||
| 言語 | ja | |||||
| 学位名 | 博士(医学) | |||||
| 学位授与機関 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 15101 | |||||
| 言語 | ja | |||||
| 学位授与機関名 | 鳥取大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2018-03-01 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲第880号 | |||||
| 権利 | ||||||
| 権利情報 | 注があるものを除き、この著作物は日本国著作権法により保護されています。 | |||||
| 情報源 | ||||||
| 関連名称 | This research was published by Yonago Acta Medica: Yoshihiro Funaki, Yasuyuki Hasegawa, Ryota Okazaki, Akira Yamasaki, Yuriko Sueda, Akihiro Yamamoto, Masaaki Yanai, Takehito Fukushima, Tomoya Harada, Haruhiko Makino and Eiji Shimizu. Resolvin E1 Inhibit | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.lib.tottori-u.ac.jp/yam/yam/yam61-1/61-1contents.html | |||||
| 関連名称 | http://www.lib.tottori-u.ac.jp/yam/yam/yam61-1/61-1contents.html | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
