WEKO3
アイテム
{"_buckets": {"deposit": "0ceb6566-56f4-47cc-8933-78628d1e65e3"}, "_deposit": {"created_by": 10, "id": "7113", "owners": [10], "pid": {"revision_id": 0, "type": "depid", "value": "7113"}, "status": "published"}, "_oai": {"id": "oai:repository.lib.tottori-u.ac.jp:00007113", "sets": ["12", "11"]}, "author_link": ["4856", "26307", "26308", "26309", "26310", "26311", "26312", "26313"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2018-03-27", "bibliographicIssueDateType": "Issued"}, "bibliographicPageStart": "18", "bibliographicVolumeNumber": "19", "bibliographic_titles": [{"bibliographic_title": "BMC GENETICS"}, {"bibliographic_title": "BMC GENETICS", "bibliographic_titleLang": "en"}]}]}, "item_10001_checkbox_27": {"attribute_name": "部局名", "attribute_value_mlt": [{"subitem_checkbox_item": ["fuzoku-shisetsu"]}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Background: The tertiary gene pool of bread wheat, to which Leymus racemosus belongs, has remained underutilized due to the current limited genomic resources of the species that constitute it. Continuous enrichment of public databases with useful information regarding these species is, therefore, needed to provide insights on their genome structures and aid successful utilization of their genes to develop improved wheat cultivars for effective management of environmental stresses. Results: We generated de novo DNA and mRNA sequence information of L. racemosus and developed 110 polymorphic PCR-based markers from the data, and to complement the PCR markers, DArT-seq genotyping was applied to develop additional 9990 SNP markers. Approximately 52% of all the markers enabled us to clearly genotype 22 wheat-L. racemosus chromosome introgression lines, and L. racemosus chromosome-specific markers were highly efficient in detailed characterization of the translocation and recombination lines analyzed. A further analysis revealed remarkable transferability of the PCR markers to three other important Triticeae perennial species: L. mollis, Psathyrostachys huashanica and Elymus ciliaris, indicating their suitability for characterizing wheat-alien chromosome introgressions carrying chromosomes of these genomes. Conclusion: The efficiency of the markers in characterizing wheat-L. racemosus chromosome introgression lines proves their reliability, and their high transferability further broadens their scope of application. This is the first report on sequencing and development of markers from L. racemosus genome and the application of DArT-seq to develop markers from a perennial wild relative of wheat, marking a paradigm shift from the seeming concentration of the technology on cultivated species. Integration of these markers with appropriate cytogenetic methods would accelerate development and characterization of wheat-alien chromosome introgression lines.", "subitem_description_type": "Other"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Bio Med Central"}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1186/s12863-018-0603-1", "subitem_relation_type_select": "DOI"}}]}, "item_10001_relation_16": {"attribute_name": "情報源", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "Edet Offiong Ukpong, Kim June-Sik, Okamoto Masanori, et al. Efficient anchoring of alien chromosome segments introgressed into bread wheat by new Leymus racemosus genome-based markers. BMC GENETICS. 2018. 19. doi:10.1186/s12863-018-0603-1"}]}]}, "item_10001_relation_17": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "https://doi.org/10.1186/s12863-018-0603-1"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1186/s12863-018-0603-1", "subitem_relation_type_select": "DOI"}}]}, "item_10001_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "(C) The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in"}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "14712156", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_text_25": {"attribute_name": "EARMAS ID", "attribute_value_mlt": [{"subitem_text_value": "8571"}]}, "item_10001_text_33": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Arid Land Research Center, Tottori University / United Graduate School of Agricultural Sciences, Tottori University"}, {"subitem_text_language": "en", "subitem_text_value": "RIKEN Center for Sustainable Resource Science"}, {"subitem_text_language": "en", "subitem_text_value": "Center for Bioscience Research and Education, Utsunomiya University"}, {"subitem_text_language": "en", "subitem_text_value": "Department of Bioscience and Bioinformatics, Kyushu Institute of Technology"}, {"subitem_text_language": "en", "subitem_text_value": "Department of Bioscience and Bioinformatics, Kyushu Institute of Technology"}, {"subitem_text_language": "en", "subitem_text_value": "International Maize and Wheat Improvement Center (CIMMYT)"}, {"subitem_text_language": "en", "subitem_text_value": "Arid Land Research Center, Tottori University / Agricultural Research Corporation (ARC)"}, {"subitem_text_language": "en", "subitem_text_value": "Arid Land Research Center, Tottori University"}]}, "item_10001_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "辻本, 壽"}, {"creatorName": "ツジモト, ヒサシ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Tsujimoto, Hisashi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4856", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "50183075", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=50183075"}, {"nameIdentifier": "100000691", "nameIdentifierScheme": "研究者総覧鳥取大学", "nameIdentifierURI": "http://researchers.adm.tottori-u.ac.jp/html/100000691_ja.html"}]}, {"creatorNames": [{"creatorName": "Edet, Offiong Ukpong", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "26307", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kim, June-Sik", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "26308", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okamoto, Masanori", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "26309", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hanada, Kousuke", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "26310", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takeda, Tomoyuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "26311", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kishii, Masahiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "26312", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Gorafi, Yasir Serag Alnor", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "26313", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2023-03-17"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "bmcg19_s12863-018-0603-1.pdf", "filesize": [{"value": "1.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "(C) The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in", "licensetype": "license_note", "mimetype": "application/pdf", "size": 1300000.0, "url": {"label": "bmcg19_s12863-018-0603-1.pdf", "url": "https://repository.lib.tottori-u.ac.jp/record/7113/files/bmcg19_s12863-018-0603-1.pdf"}, "version_id": "c829a82c-b4dd-4a49-8aae-747220bee093"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Leymus racemosus (1)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Molecular markers (2)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "DArT-seq (3)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Genome sequencing (4)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Chromosome introgression lines (5)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Salinity stress (6)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Triticeae (7)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Leymus racemosus (1)", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Molecular markers (2)", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "DArT-seq (3)", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Genome sequencing (4)", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Chromosome introgression lines (5)", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Salinity stress (6)", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Triticeae (7)", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Efficient anchoring of alien chromosome segments introgressed into bread wheat by new Leymus racemosus genome-based markers", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Efficient anchoring of alien chromosome segments introgressed into bread wheat by new Leymus racemosus genome-based markers", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "10", "path": ["12", "11"], "permalink_uri": "https://repository.lib.tottori-u.ac.jp/records/7113", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2022-09-08"}, "publish_date": "2022-09-08", "publish_status": "0", "recid": "7113", "relation": {}, "relation_version_is_last": true, "title": ["Efficient anchoring of alien chromosome segments introgressed into bread wheat by new Leymus racemosus genome-based markers"], "weko_shared_id": -1}
Efficient anchoring of alien chromosome segments introgressed into bread wheat by new Leymus racemosus genome-based markers
https://repository.lib.tottori-u.ac.jp/records/7113
https://repository.lib.tottori-u.ac.jp/records/71137b35590b-47c7-4461-b513-8e9e9da9692e
名前 / ファイル | ライセンス | アクション |
---|---|---|
![]() |
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2022-09-08 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Efficient anchoring of alien chromosome segments introgressed into bread wheat by new Leymus racemosus genome-based markers | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | Leymus racemosus (1) | |||||
キーワード | ||||||
主題 | Molecular markers (2) | |||||
キーワード | ||||||
主題 | DArT-seq (3) | |||||
キーワード | ||||||
主題 | Genome sequencing (4) | |||||
キーワード | ||||||
主題 | Chromosome introgression lines (5) | |||||
キーワード | ||||||
主題 | Salinity stress (6) | |||||
キーワード | ||||||
主題 | Triticeae (7) | |||||
キーワード | ||||||
言語 | en | |||||
主題 | Leymus racemosus (1) | |||||
キーワード | ||||||
言語 | en | |||||
主題 | Molecular markers (2) | |||||
キーワード | ||||||
言語 | en | |||||
主題 | DArT-seq (3) | |||||
キーワード | ||||||
言語 | en | |||||
主題 | Genome sequencing (4) | |||||
キーワード | ||||||
言語 | en | |||||
主題 | Chromosome introgression lines (5) | |||||
キーワード | ||||||
言語 | en | |||||
主題 | Salinity stress (6) | |||||
キーワード | ||||||
言語 | en | |||||
主題 | Triticeae (7) | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
著者 |
辻本, 壽
× 辻本, 壽× Edet, Offiong Ukpong× Kim, June-Sik× Okamoto, Masanori× Hanada, Kousuke× Takeda, Tomoyuki× Kishii, Masahiro× Gorafi, Yasir Serag Alnor |
|||||
著者所属(英) | ||||||
en | ||||||
Arid Land Research Center, Tottori University / United Graduate School of Agricultural Sciences, Tottori University | ||||||
著者所属(英) | ||||||
en | ||||||
RIKEN Center for Sustainable Resource Science | ||||||
著者所属(英) | ||||||
en | ||||||
Center for Bioscience Research and Education, Utsunomiya University | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Bioscience and Bioinformatics, Kyushu Institute of Technology | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Bioscience and Bioinformatics, Kyushu Institute of Technology | ||||||
著者所属(英) | ||||||
en | ||||||
International Maize and Wheat Improvement Center (CIMMYT) | ||||||
著者所属(英) | ||||||
en | ||||||
Arid Land Research Center, Tottori University / Agricultural Research Corporation (ARC) | ||||||
著者所属(英) | ||||||
en | ||||||
Arid Land Research Center, Tottori University | ||||||
抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Background: The tertiary gene pool of bread wheat, to which Leymus racemosus belongs, has remained underutilized due to the current limited genomic resources of the species that constitute it. Continuous enrichment of public databases with useful information regarding these species is, therefore, needed to provide insights on their genome structures and aid successful utilization of their genes to develop improved wheat cultivars for effective management of environmental stresses. Results: We generated de novo DNA and mRNA sequence information of L. racemosus and developed 110 polymorphic PCR-based markers from the data, and to complement the PCR markers, DArT-seq genotyping was applied to develop additional 9990 SNP markers. Approximately 52% of all the markers enabled us to clearly genotype 22 wheat-L. racemosus chromosome introgression lines, and L. racemosus chromosome-specific markers were highly efficient in detailed characterization of the translocation and recombination lines analyzed. A further analysis revealed remarkable transferability of the PCR markers to three other important Triticeae perennial species: L. mollis, Psathyrostachys huashanica and Elymus ciliaris, indicating their suitability for characterizing wheat-alien chromosome introgressions carrying chromosomes of these genomes. Conclusion: The efficiency of the markers in characterizing wheat-L. racemosus chromosome introgression lines proves their reliability, and their high transferability further broadens their scope of application. This is the first report on sequencing and development of markers from L. racemosus genome and the application of DArT-seq to develop markers from a perennial wild relative of wheat, marking a paradigm shift from the seeming concentration of the technology on cultivated species. Integration of these markers with appropriate cytogenetic methods would accelerate development and characterization of wheat-alien chromosome introgression lines. | |||||
書誌情報 |
BMC GENETICS en : BMC GENETICS 巻 19, p. 18, 発行日 2018-03-27 |
|||||
出版者 | ||||||
出版者 | Bio Med Central | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 14712156 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1186/s12863-018-0603-1 | |||||
権利 | ||||||
権利情報 | (C) The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in | |||||
情報源 | ||||||
関連名称 | Edet Offiong Ukpong, Kim June-Sik, Okamoto Masanori, et al. Efficient anchoring of alien chromosome segments introgressed into bread wheat by new Leymus racemosus genome-based markers. BMC GENETICS. 2018. 19. doi:10.1186/s12863-018-0603-1 | |||||
関連サイト | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1186/s12863-018-0603-1 | |||||
関連名称 | https://doi.org/10.1186/s12863-018-0603-1 | |||||
著者版フラグ | ||||||
出版タイプ | VoR |