| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2021-09-08 |
| タイトル |
|
|
タイトル |
CD44 standard isoform is involved in maintenance of cancer stem cells of a hepatocellular carcinoma cell line |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
cancer stem cell |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
CD44 |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
hepatocellular carcinoma |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
NOTCH3 |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
oxidative stress |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
cancer stem cell |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
CD44 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
hepatocellular carcinoma |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
NOTCH3 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
oxidative stress |
| 資源タイプ |
|
|
資源タイプ |
journal article |
| 著者 |
土谷, 博之
網崎, 正孝
坂部, 友彦
汐田, 剛史
Asai, Ryoma
Makimoto, Kazuki
Takenaga, Ai
Hoi, Shotaro
Koyama, Shigemi
|
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University / Faculty of Medicine, Division of Surgical Oncology, Department of Surgery, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Faculty of Medicine, Division of Organ Pathology, Department of Pathology, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University |
| 抄録 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer death worldwide. Cancer stem cells (CSCs) have attracted attention as a novel therapeutic target for cancer because they play important roles in the development and aggravation of cancer. CD44 is expressed as a standard isoform (CD44s) and several variant isoforms. CD44v is a major isoform expressed on CSCs of a variety of tumors and has been extensively studied. However, HCC tissues dominantly express CD44s, whose function in CSCs remains unclear. In the present study, we investigated the roles of CD44s in CSCs of HCC. Knock-out of the CD44 gene in HuH7 HCC cells on which only CD44s is expressed resulted in decreased spheroid formation and increased drug sensitivity. The expression of CSC marker genes, including CD133 and EpCAM, was significantly downregulated in the spheroids of CD44-deficient cells compared with those in the spheroids of HuH7 cells. In addition, CD44 deficiency impaired antioxidant capacity, concomitant with downregulation of glutathione peroxidase 1 (GPX1) and thioredoxin. Because GPX1 uses the reduced form of glutathione (GSH) to regenerate oxidized cellular components, GSH levels were significantly increased in the CD44-deficient cells. We also found that NOTCH3 and its target genes were downregulated in the spheroids of CD44-deficient cells. NOTCH3 expression in HCC tissues was significantly increased compared with that in adjacent nontumor liver tissues and was correlated with CD44 expression. These results suggest that CD44s is involved in maintenance of CSCs in a HCC cell line, possibly through the NOTCH3 signaling pathway. |
| 書誌情報 |
CANCER MEDICINE
en : CANCER MEDICINE
巻 8,
号 2,
p. 773-782,
発行日 2019-02
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| 出版者 |
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|
出版者 |
WILEY |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
20457634 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1002/cam4.1968 |
| 権利 |
|
|
権利情報 |
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. © 2019 The Authors. Cancer Medicine published by John |
| 情報源 |
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|
関連名称 |
Asai Ryoma, Tsuchiya Hiroyuki, Amisaki Masataka, et al. CD44 standard isoform is involved in maintenance of cancer stem cells of a hepatocellular carcinoma cell line. CANCER MEDICINE. 2019. 8(2). 773-782. doi:10.1002/cam4.1968 |
| 関連サイト |
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|
識別子タイプ |
URI |
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|
関連識別子 |
https://onlinelibrary.wiley.com/doi/full/10.1002/cam4.1968 |
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|
関連名称 |
https://onlinelibrary.wiley.com/doi/full/10.1002/cam4.1968 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
cm8(2)_773.pdf (550.8 kB)
