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Application of Bio-Based Wrinkled Surfaces as Cell Culture Scaffolds
https://repository.lib.tottori-u.ac.jp/records/7157
https://repository.lib.tottori-u.ac.jp/records/7157e79c7846-5d20-4458-aa36-528b29582eb1
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ci2(2)_15.pdf (810.4 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2019-08-06 | |||||
| タイトル | ||||||
| タイトル | Application of Bio-Based Wrinkled Surfaces as Cell Culture Scaffolds | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | chitosan | |||||
| キーワード | ||||||
| 主題 | surface wrinkling | |||||
| キーワード | ||||||
| 主題 | skin layer | |||||
| キーワード | ||||||
| 主題 | horseradish peroxidase | |||||
| キーワード | ||||||
| 主題 | cell culture substrate | |||||
| キーワード | ||||||
| 主題 | 3T3 cell | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | chitosan | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | surface wrinkling | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | skin layer | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | horseradish peroxidase | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | cell culture substrate | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | 3T3 cell | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Izawa, Hironori
× Izawa, Hironori× Okuda, Noriko× Yonemura, Tomoe× Kuroda, Kohei× Ochi, Kosuke× Ifuku, Shinsuke× Morimoto, Minoru× Saimoto, Hiroyuki× Noda, Mayuko× Azuma, Kazuo× Okamoto, Yoshiharu× Ito, Norihiko |
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| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Graduate School of Engineering, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Graduate School of Engineering, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Graduate School of Engineering, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Veterinary Clinical Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Veterinary Clinical Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Graduate School of Engineering, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Instrumental Analysis, Research Center for Bioscience and Technology, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Graduate School of Engineering, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Veterinary Clinical Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Veterinary Clinical Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Veterinary Clinical Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Veterinary Clinical Medicine, Tottori University | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Microscopic surface architectures that can be easily manufactured have been in demand as mechano-structural cues for tissue engineering. Microscopic surface reliefs synthesized by wrinkling were expected as cell culture scaffolds for cell proliferation, control of cellular alignment and differentiation, and spheroid generation. We previously developed bio-based wrinkled films prepared via lignification-mimetic reactions and drying. Although these films are expected as a candidate for cell culture scaffolds, stability and morphology of the wrinkled surfaces in aqueous buffer solutions were not explored. Here, we investigate the surface morphologies of the wrinkled films in phosphate-buffered saline, and their application to 3T3 cell culture. The wrinkled film prepared with the immersion treatment at 40 °C maintained its wrinkled structure in phosphate-buffered saline even after five days, although the wrinkles were broadened by hydration of the skin layer. Interestingly, higher cell numbers were observed in the 3T3 cell culture using the wrinkled film than using flat film with the same surface composition. In addition, the high biocompatibility of the wrinkled film was confirmed by in vivo experiments. These results strongly encourage application of the wrinkled film as a mechano-structural cue. Studies of the advanced applications for the wrinkled films are now in progress. | |||||
| 書誌情報 |
Colloids Interfaces en : Colloids Interfaces 巻 2, 号 2, p. 15, 発行日 2018-03-26 |
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| 出版者 | ||||||
| 出版者 | MDPI AG | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 25045377 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.3390/colloids2020015 | |||||
| 権利 | ||||||
| 権利情報 | © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | |||||
| 情報源 | ||||||
| 関連名称 | IZAWA, Hironori, et al. Application of bio-based wrinkled surfaces as cell culture scaffolds. Colloids and Interfaces, 2018, 2.2: 15. | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.mdpi.com/2504-5377/2/2/15 | |||||
| 関連名称 | https://www.mdpi.com/2504-5377/2/2/15 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
