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Developing a mouse model of acute encephalopathy using low-dose lipopolysaccharide injection and hyperthermia treatment
https://repository.lib.tottori-u.ac.jp/records/7193
https://repository.lib.tottori-u.ac.jp/records/7193ded3d615-3bb4-488c-9a23-6d5668c3bf74
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ebm244(9)_743.pdf (1.4 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2020-01-16 | |||||
| タイトル | ||||||
| タイトル | Developing a mouse model of acute encephalopathy using low-dose lipopolysaccharide injection and hyperthermia treatment | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | Hyperthermia | |||||
| キーワード | ||||||
| 主題 | lipopolysaccharide | |||||
| キーワード | ||||||
| 主題 | seizure | |||||
| キーワード | ||||||
| 主題 | blood–brain barrier | |||||
| キーワード | ||||||
| 主題 | vasogenic edema | |||||
| キーワード | ||||||
| 主題 | acute encephalopathy | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | Hyperthermia | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | lipopolysaccharide | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | seizure | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | blood–brain barrier | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | vasogenic edema | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | acute encephalopathy | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Kurata, Hirofumi
× Kurata, Hirofumi× Saito, Kengo× Kawashima, Fumiaki× Ikenari, Takuya× Oguri, Masayoshi× Saito, Yoshiaki× Maegaki, Yoshihiro× Mori, Tetsuji |
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| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Biological Regulation, School of Health Science, Faculty of Medicine, Tottori University / Division of Child Neurology, Department of Brain and Neurosciences, Tottori University / Department of Pediatrics, National Hospital Organization, Kumamoto Saishunso National Hospital | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Biological Regulation, School of Health Science, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Biological Regulation, School of Health Science, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Biological Regulation, School of Health Science, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Pathobiological Science and Technology, School of Health Science, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Child Neurology, Department of Brain and Neurosciences, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Child Neurology, Department of Brain and Neurosciences, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Biological Regulation, School of Health Science, Faculty of Medicine, Tottori University | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Acute encephalopathy (AE) is mainly reported in East Asia and, in most cases, results from pediatric viral infections, leading to fever, seizure, and loss of consciousness. Cerebral edema is the most important pathological symptom of AE. At present, AE is classified into four categories based on clinical and pathophysiological features, and cytokine storm-induced AE is the severest among them. The pathogenesis of AE is currently unclear; this can be attributed to the lack of a simple and convenient animal model for research. Here, we hypothesized that the induction of systemic inflammation using lipopolysaccharide (LPS) injection followed by hyperthermia (HT) treatment can be used to develop an animal model of cytokine storm-induced AE. Postnatal eight-day-old mouse pups were intraperitoneally injected with low-dose LPS (50 or 100 µg/kg) followed by HT treatment (41.5°C, 30 min). Histological analysis of their brains was subsequently performed. Fluorescein isothiocyanate assay combined with immunohistochemistry was used to elucidate blood–brain barrier (BBB) disruption. LPS (100 µg/kg) injection followed by HT treatment increased BBB permeability in the cerebral cortex and induced microglial activation. Astrocytic clasmatodendrosis was also evident. The brains of some pups exhibited small ischemic lesions, particularly in the cerebral cortex. Our results indicate that a low-dose LPS injection followed by HT treatment can produce symptoms of cytokine storm-induced AE, which is observed in diseases, such as acute necrotizing encephalopathy and hemorrhagic shock and encephalopathy syndrome. Thus, this mouse model can help to elucidate the pathogenetic mechanisms underlying AE. | |||||
| 書誌情報 |
Experimental biology and medicine en : Experimental biology and medicine 巻 244, 号 9, p. 743-751, 発行日 2019-05-02 |
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| 雑誌内区分 | ||||||
| 値 | Research Article | |||||
| 出版者 | ||||||
| 出版者 | Society for Experimental Biology and Medicine | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 15353702 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11503891 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1177/1535370219846497 | |||||
| 権利 | ||||||
| 権利情報 | Copyright © 2019 by the Society for Experimental Biology and Medicine. | |||||
| 情報源 | ||||||
| 関連名称 | This research was published by the Society for Experimental Biology and Medicine: Kurata, H., Saito, K., Kawashima, F., Ikenari, T., Oguri, M., Saito, Y., … Mori, T. (2019). Developing a mouse model of acute encephalopathy using low-dose lipopolysaccharid | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://journals.sagepub.com/doi/abs/10.1177/1535370219846497 | |||||
| 関連名称 | https://journals.sagepub.com/doi/abs/10.1177/1535370219846497 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://repository.lib.tottori-u.ac.jp/6527 | |||||
| 関連名称 | https://repository.lib.tottori-u.ac.jp/6527 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
