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Frequent Transposition of Multiple Insertion Sequences in Geobacillus kaustophilus HTA426
https://repository.lib.tottori-u.ac.jp/records/7196
https://repository.lib.tottori-u.ac.jp/records/7196cf721ec0-13b2-4bd0-bf9b-2fba95da905a
| 名前 / ファイル | ライセンス | アクション |
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fm_fmicb.2021.650461.pdf (1.9 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2022-07-28 | |||||
| タイトル | ||||||
| タイトル | Frequent Transposition of Multiple Insertion Sequences in Geobacillus kaustophilus HTA426 | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | extra-cytoplasmic function sigma factor | |||||
| キーワード | ||||||
| 主題 | IS4 | |||||
| キーワード | ||||||
| 主題 | IS701 | |||||
| キーワード | ||||||
| 主題 | IS1634 | |||||
| キーワード | ||||||
| 主題 | ISLre2 | |||||
| キーワード | ||||||
| 主題 | stress-induced transposition | |||||
| キーワード | ||||||
| 主題 | thermophile | |||||
| キーワード | ||||||
| 主題 | transposable element | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | extra-cytoplasmic function sigma factor | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | IS4 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | IS701 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | IS1634 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | ISLre2 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | stress-induced transposition | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | thermophile | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | transposable element | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
鈴木, 宏和
× 鈴木, 宏和× 大城, 隆× Taketani, Tatsunari× Tanabiki, Misaki× Ohara, Misaki× Kobayashi, Jyumpei |
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| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Faculty of Engineering, Tottori University / Center for Research on Green Sustainable Chemistry, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Engineering, Graduate School of Sustainability Science, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Engineering, Graduate School of Sustainability Science, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Faculty of Engineering, Tottori University / Center for Research on Green Sustainable Chemistry, Tottori University | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Geobacillus kaustophilus HTA426 is a thermophilic bacterium whose genome harbors numerous insertion sequences (IS). This study was initially conducted to generate mutant genes for thermostable T7 RNA polymerase in G. kaustophilus; however, relevant experiments unexpectedly identified that the organism transposed multiple IS elements and produced derivative cells that expressed a silent gene via transposition. The transposed elements were diverse and included members of the IS4, IS701, IS1634, and ISLre2 families. The transposition was relatively active at elevated temperatures and generated 4–9 bp of direct repeats at insertion sites. Transposition was more frequent in proliferative cells than in stationary cells but was comparable between both cells when sigX, which encodes an extra-cytoplasmic function sigma factor, was forcibly expressed. Southern blot analysis indicated that IS transposition occurred under growth inhibitory conditions by diverse stressors; however, IS transposition was not detected in cells that were cultured under growth non-inhibitory conditions. These observations suggest that G. kaustophilus enhances IS transposition via sigX-dependent stress responses when proliferative cells were prevented from active propagation. Considering Geobacillus spp. are highly adaptive bacteria that are remarkably distributed in diverse niches, it is possible that these organisms employ IS transposition for environmental adaptation via genetic diversification. Thus, this study provides new insights into adaptation strategies of Geobacillus spp. along with implications for strong codependence between mobile genetic elements and highly adaptive bacteria for stable persistence and evolutionary diversification, respectively. This is also the first report to reveal active IS elements at elevated temperatures in thermophiles and to suggest a sigma factor that governs IS transposition. | |||||
| 書誌情報 |
FRONTIERS IN MICROBIOLOGY en : FRONTIERS IN MICROBIOLOGY 巻 12, 発行日 2021-03-24 |
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| 出版者 | ||||||
| 出版者 | Frontiers Media | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1664302X | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.3389/fmicb.2021.650461 | |||||
| 権利 | ||||||
| 権利情報 | (C) 2021 Suzuki, Taketani, Tanabiki, Ohara, Kobayashi and Ohshiro. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided | |||||
| 情報源 | ||||||
| 関連名称 | Suzuki Hirokazu, Taketani Tatsunari, Tanabiki Misaki, et al. Frequent Transposition of Multiple Insertion Sequences in Geobacillus kaustophilus HTA426. FRONTIERS IN MICROBIOLOGY. 2021. 12. doi:10.3389/fmicb.2021.650461 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.frontiersin.org/articles/10.3389/fmicb.2021.650461 | |||||
| 関連名称 | https://www.frontiersin.org/articles/10.3389/fmicb.2021.650461 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
