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  1. 学部学科区分一覧
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  2. 学術雑誌論文

Frequent Transposition of Multiple Insertion Sequences in Geobacillus kaustophilus HTA426

https://repository.lib.tottori-u.ac.jp/records/7196
https://repository.lib.tottori-u.ac.jp/records/7196
cf721ec0-13b2-4bd0-bf9b-2fba95da905a
名前 / ファイル ライセンス アクション
fm_fmicb.2021.650461.pdf fm_fmicb.2021.650461.pdf (1.9 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2022-07-28
タイトル
タイトル Frequent Transposition of Multiple Insertion Sequences in Geobacillus kaustophilus HTA426
言語 en
言語
言語 eng
キーワード
主題 extra-cytoplasmic function sigma factor
キーワード
主題 IS4
キーワード
主題 IS701
キーワード
主題 IS1634
キーワード
主題 ISLre2
キーワード
主題 stress-induced transposition
キーワード
主題 thermophile
キーワード
主題 transposable element
キーワード
言語 en
主題 extra-cytoplasmic function sigma factor
キーワード
言語 en
主題 IS4
キーワード
言語 en
主題 IS701
キーワード
言語 en
主題 IS1634
キーワード
言語 en
主題 ISLre2
キーワード
言語 en
主題 stress-induced transposition
キーワード
言語 en
主題 thermophile
キーワード
言語 en
主題 transposable element
資源タイプ
資源タイプ journal article
著者 鈴木, 宏和

× 鈴木, 宏和

WEKO 4922
e-Rad 80462696
研究者総覧鳥取大学 100001258

鈴木, 宏和

ja-Kana スズキ, ヒロカズ

en Suzuki, Hirokazu

Search repository
大城, 隆

× 大城, 隆

WEKO 4819
e-Rad 00233106
研究者総覧鳥取大学 100000478

大城, 隆

ja-Kana オオシロ, タカシ

en Ohshiro, Takashi

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Taketani, Tatsunari

× Taketani, Tatsunari

WEKO 26645

en Taketani, Tatsunari

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Tanabiki, Misaki

× Tanabiki, Misaki

WEKO 26646

en Tanabiki, Misaki

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Ohara, Misaki

× Ohara, Misaki

WEKO 26647

en Ohara, Misaki

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Kobayashi, Jyumpei

× Kobayashi, Jyumpei

WEKO 26648

en Kobayashi, Jyumpei

Search repository
著者所属(英)
言語 en
値 Faculty of Engineering, Tottori University / Center for Research on Green Sustainable Chemistry, Tottori University
著者所属(英)
言語 en
値 Department of Engineering, Graduate School of Sustainability Science, Tottori University
著者所属(英)
言語 en
値 Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University
著者所属(英)
言語 en
値 Department of Engineering, Graduate School of Sustainability Science, Tottori University
著者所属(英)
言語 en
値 Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University
著者所属(英)
言語 en
値 Faculty of Engineering, Tottori University / Center for Research on Green Sustainable Chemistry, Tottori University
抄録
内容記述タイプ Other
内容記述 Geobacillus kaustophilus HTA426 is a thermophilic bacterium whose genome harbors numerous insertion sequences (IS). This study was initially conducted to generate mutant genes for thermostable T7 RNA polymerase in G. kaustophilus; however, relevant experiments unexpectedly identified that the organism transposed multiple IS elements and produced derivative cells that expressed a silent gene via transposition. The transposed elements were diverse and included members of the IS4, IS701, IS1634, and ISLre2 families. The transposition was relatively active at elevated temperatures and generated 4–9 bp of direct repeats at insertion sites. Transposition was more frequent in proliferative cells than in stationary cells but was comparable between both cells when sigX, which encodes an extra-cytoplasmic function sigma factor, was forcibly expressed. Southern blot analysis indicated that IS transposition occurred under growth inhibitory conditions by diverse stressors; however, IS transposition was not detected in cells that were cultured under growth non-inhibitory conditions. These observations suggest that G. kaustophilus enhances IS transposition via sigX-dependent stress responses when proliferative cells were prevented from active propagation. Considering Geobacillus spp. are highly adaptive bacteria that are remarkably distributed in diverse niches, it is possible that these organisms employ IS transposition for environmental adaptation via genetic diversification. Thus, this study provides new insights into adaptation strategies of Geobacillus spp. along with implications for strong codependence between mobile genetic elements and highly adaptive bacteria for stable persistence and evolutionary diversification, respectively. This is also the first report to reveal active IS elements at elevated temperatures in thermophiles and to suggest a sigma factor that governs IS transposition.
書誌情報 FRONTIERS IN MICROBIOLOGY
en : FRONTIERS IN MICROBIOLOGY

巻 12, 発行日 2021-03-24
出版者
出版者 Frontiers Media
ISSN
収録物識別子タイプ ISSN
収録物識別子 1664302X
DOI
関連タイプ isIdenticalTo
識別子タイプ DOI
関連識別子 10.3389/fmicb.2021.650461
権利
権利情報 (C) 2021 Suzuki, Taketani, Tanabiki, Ohara, Kobayashi and Ohshiro. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided
情報源
関連名称 Suzuki Hirokazu, Taketani Tatsunari, Tanabiki Misaki, et al. Frequent Transposition of Multiple Insertion Sequences in Geobacillus kaustophilus HTA426. FRONTIERS IN MICROBIOLOGY. 2021. 12. doi:10.3389/fmicb.2021.650461
関連サイト
識別子タイプ URI
関連識別子 https://www.frontiersin.org/articles/10.3389/fmicb.2021.650461
関連名称 https://www.frontiersin.org/articles/10.3389/fmicb.2021.650461
著者版フラグ
出版タイプ VoR
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