| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2019-08-01 |
| タイトル |
|
|
タイトル |
Infection of endotheliotropic human cytomegalovirus of trabecular meshwork cells |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
cytomegalovirus |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
corneal endothelial cells |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
trabecular meshwork |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
secondary glaucoma |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
endotheliitis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
cytomegalovirus |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
corneal endothelial cells |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
trabecular meshwork |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
secondary glaucoma |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
endotheliitis |
| 資源タイプ |
|
|
資源タイプ |
journal article |
| 著者 |
Shimizu, Daisuke
Miyazaki, Dai
Shimizu, Yumiko
Hosogai, Mayumi
Kosugi, Isao
Inoue, Yoshitsugu
Shimizu, Daisuke
Shimizu, Yumiko
Hosogai, Mayumi
Kosugi, Isao
|
| 著者所属 |
|
|
値 |
Ophthalmology and Visual Science, Tottori University Faculty of Medicine / Department of Ophthalmology, Chiba University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Ophthalmology and Visual Science, Tottori University Faculty of Medicine |
| 著者所属 |
|
|
値 |
Ophthalmology and Visual Science, Tottori University Faculty of Medicine |
| 著者所属 |
|
|
値 |
Department of Ophthalmology, Gunma University Graduate School of Medicine |
| 著者所属 |
|
|
値 |
Regenerative and Infectious Pathology, Hamamatsu University, School of Medicine |
| 著者所属 |
|
|
値 |
Ophthalmology and Visual Science, Tottori University Faculty of Medicine |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Ophthalmology and Visual Science, Tottori University Faculty of Medicine / Department of Ophthalmology, Chiba University Graduate School of Medicine |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Ophthalmology and Visual Science, Tottori University Faculty of Medicine |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Ophthalmology and Visual Science, Tottori University Faculty of Medicine |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Department of Ophthalmology, Gunma University Graduate School of Medicine |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Regenerative and Infectious Pathology, Hamamatsu University, School of Medicine |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Ophthalmology and Visual Science, Tottori University Faculty of Medicine |
| 抄録 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Purpose: Human cytomegalovirus (HCMV) infections can cause endotheliitis which is associated with an elevation of the intraocular pressure (IOP). However, the mechanism of the IOP elevation has not been determined. The purpose of this study was to determine whether HCMV strains which are capable of infecting corneal endothelial cells can also replicate, induce anti-viral responses, and can reorganize the actin cytoskeleton in trabecular meshwork cells. Study design: Experimental study design Methods: Cultured primary human trabecular meshwork cells (HTMCs) were infected with the Towne or TB40/E strains of HCMV. TB40/E is trophic for vascular endothelial and corneal endothelial cells. Real-time PCR, western blot, and fluorescent immunostaining have been used to determine whether HCMV-infected HTMCs will support the expression of viral mRNA and protein, allow viral replication, and elicit anti-viral host responses. We also determined whether lytic replication was present after an HCMV infection. Results: HCMV infection led to the expression of viral mRNA and proteins of IE1, glycoprotein B(gB), and pp65. TB40/E infection induced interferon-β, a sign of host anti-viral immune response and MCP-1. Together with the induction of the regulators of actin cytoskeleton, myosin phosphatase Rho interacting protein (MPRIP) and monocyte chemotactic protein-1 (MCP-1), TB40/E induced a high level of expression of viral proteins, including IE1, gB, and pp65 as well as actin stress fiber formation, and achieved pathogenically high viral titers. Conclusions: Human trabecular meshwork cells support the replication of endotheliotropic TB40/E strain of HCMV which indicates that this strain may have high virulence for trabecular meshwork. |
| 書誌情報 |
Japanese Journal of Ophthalmology
en : Japanese Journal of Ophthalmology
巻 62,
号 6,
p. 667-676,
発行日 2018-09-06
|
| 出版者 |
|
|
出版者 |
Springer Japan |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
00215155 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00691177 |
| DOI |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1007/s10384-018-0618-1 |
| 権利 |
|
|
権利情報 |
© Japanese Ophthalmological Society 2018 |
| 情報源 |
|
|
|
関連名称 |
Japanese Journal of Ophthalmology. 2018, 62(6), 667-676 This is a post-peer-review, pre-copyedit version of an article published in Japanese Journal of Ophthalmology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10384 |
| 関連サイト |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
https://link.springer.com/article/10.1007%2Fs10384-018-0618-1 |
|
|
関連名称 |
https://link.springer.com/article/10.1007%2Fs10384-018-0618-1 |
| 著者版フラグ |
|
|
出版タイプ |
AM |
jjo62(6)_667.pdf (1.2 MB)
