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An α-synuclein decoy peptide prevents cytotoxic α-synuclein aggregation caused by fatty acid binding protein 3
https://repository.lib.tottori-u.ac.jp/records/7245
https://repository.lib.tottori-u.ac.jp/records/7245c2e3d82c-3a9f-4c8c-815d-aafbcf3ca273
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
jbc296_j.jbc.2021.100663.pdf (5.0 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2022-06-30 | |||||
| タイトル | ||||||
| タイトル | An α-synuclein decoy peptide prevents cytotoxic α-synuclein aggregation caused by fatty acid binding protein 3 | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
フクイ, ナオヤ
× フクイ, ナオヤ× 本郷, 邦広× 溝端, 知宏× 河田, 康志× Fukui, Naoya× Yamamoto, Hanae× Miyabe, Moe× Aoyama, Yuki× Kawahata, Ichiro× Yabuki, Yasushi× Shinoda, Yasuharu× Fukunaga, Kohji |
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| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Chemistry and Biotechnology, Faculty of Engineering/Graduate School of Engineering, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Chemistry and Biotechnology, Faculty of Engineering/Graduate School of Engineering, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Chemistry and Biotechnology, Faculty of Engineering/Graduate School of Engineering, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Chemistry and Biotechnology, Faculty of Engineering/Graduate School of Engineering, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Chemistry and Biotechnology, Faculty of Engineering/Graduate School of Engineering, Tottori University / Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University / Center for Research on Green Sustainable Chemistry, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Chemistry and Biotechnology, Faculty of Engineering/Graduate School of Engineering, Tottori University / Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University / Center for Research on Green Sustainable Chemistry, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Tohoku University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Tohoku University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Tohoku University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Tohoku University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Chemistry and Biotechnology, Faculty of Engineering/Graduate School of Engineering, Tottori University / Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University / Center for Research on Green Sustainable Chemistry, Tottori University | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | α-synuclein (αSyn) is a protein known to form intracellular aggregates during the manifestation of Parkinson’s disease. Previously, it was shown that αSyn aggregation was strongly suppressed in the midbrain region of mice that did not possess the gene encoding the lipid transport protein fatty acid binding protein 3 (FABP3). An interaction between these two proteins was detected in vitro, suggesting that FABP3 may play a role in the aggregation and deposition of αSyn in neurons. In order to characterize the molecular mechanisms that underlie the interactions between FABP3 and αSyn that modulate the cellular accumulation of the latter, in this report, we used in vitro fluorescence assays combined with fluorescence microscopy, transmission electron microscopy, and quartz crystal microbalance assays to characterize in detail the process and consequences of FABP3-αSyn interaction. We demonstrated that binding of FABP3 to αSyn results in changes in the aggregation mechanism of the latter; specifically, a suppression of fibrillar forms of αSyn, and also the production of aggregates with an enhanced cytotoxicity toward mice neuro2A cells. Since this interaction involved the C-terminal sequence region of αSyn, we tested a peptide derived from this region of αSyn (αSynP130-140) as a decoy to prevent the FABP3-αSyn interaction. We observed that the peptide competitively inhibited binding of αSyn to FABP3 in vitro and in cultured cells. We propose that administration of αSynP130-140 might be used to prevent the accumulation of toxic FABP3-αSyn oligomers in cells, thereby preventing the progression of Parkinson’s disease. | |||||
| 書誌情報 |
Journal of Biological Chemistry en : Journal of Biological Chemistry 巻 296, 発行日 2021-04-20 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00219258 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA1202441X | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.jbc.2021.100663 | |||||
| 権利 | ||||||
| 権利情報 | © 2021 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology. http://creativecommons.org/licenses/by/4.0/ | |||||
| 情報源 | ||||||
| 関連名称 | Fukui, Naoya. Yamamoto, Hanae. Miyabe, Moe. et al. An α-synuclein decoy peptide prevents cytotoxic α-synuclein aggregation caused by fatty acid binding protein 3. Journal of Biological Chemistry. 296, 100663. 2021-04-20. | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.sciencedirect.com/science/article/pii/S0021925821004518 | |||||
| 関連名称 | https://www.sciencedirect.com/science/article/pii/S0021925821004518 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| EISSN | ||||||
| 値 | 1083351X | |||||
