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  1. 学部学科区分一覧
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Phosphorylation of SNAP-23 at Ser95 causes a structural alteration and negatively regulates Fc receptor-mediated phagosome formation and maturation in macrophages

https://repository.lib.tottori-u.ac.jp/records/7308
https://repository.lib.tottori-u.ac.jp/records/7308
798d8b66-0cb2-45ff-91cc-93e9aae96c39
名前 / ファイル ライセンス アクション
mbc29(14)_1753.pdf mbc29(14)_1753.pdf (3.6 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2021-11-25
タイトル
タイトル Phosphorylation of SNAP-23 at Ser95 causes a structural alteration and negatively regulates Fc receptor-mediated phagosome formation and maturation in macrophages
言語 en
言語
言語 eng
資源タイプ
資源タイプ journal article
著者 櫻井, 千恵

× 櫻井, 千恵

WEKO 4929
e-Rad 10589724
研究者総覧鳥取大学 100001302

櫻井, 千恵

ja-Kana サクライ, チエ

en Sakurai, Chiye

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初沢, 清隆

× 初沢, 清隆

WEKO 4750
e-Rad 20256655
研究者総覧鳥取大学 100001086

初沢, 清隆

ja-Kana ハツザワ, キヨタカ

en Hatsuzawa, Kiyotaka

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Itakura, Makoto

× Itakura, Makoto

WEKO 27146

en Itakura, Makoto

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Kinoshita, Daiki

× Kinoshita, Daiki

WEKO 27147

en Kinoshita, Daiki

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Arai, Seisuke

× Arai, Seisuke

WEKO 27148

en Arai, Seisuke

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Hashimoto, Hitoshi

× Hashimoto, Hitoshi

WEKO 27149

en Hashimoto, Hitoshi

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Wada, Ikuo

× Wada, Ikuo

WEKO 27150

en Wada, Ikuo

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著者所属(英)
言語 en
値 Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University / Department of Cell Science, Institute of Biomedical Science, Fukushima Medical University School of Medicine
著者所属(英)
言語 en
値 Department of Biochemistry, Kitasato University School of Medicine
著者所属(英)
言語 en
値 Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University
著者所属(英)
言語 en
値 Department of Cell Science, Institute of Biomedical Science, Fukushima Medical University School of Medicine
著者所属(英)
言語 en
値 Department of Cell Science, Institute of Biomedical Science, Fukushima Medical University School of Medicine
著者所属(英)
言語 en
値 Department of Cell Science, Institute of Biomedical Science, Fukushima Medical University School of Medicine
著者所属(英)
言語 en
値 Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University / Department of Cell Science, Institute of Biomedical Science, Fukushima Medical University School of Medicine
抄録
内容記述タイプ Other
内容記述 SNAP-23 is a plasma membrane-localized soluble N-ethylmaleimide–sensitive factor attachment protein receptors (SNARE) involved in Fc receptor (FcR)-mediated phagocytosis. However, the regulatory mechanism underlying its function remains elusive. Using phosphorylation-specific antibodies, SNAP-23 was found to be phosphorylated at Ser95 in macrophages. To understand the role of this phosphorylation, we established macrophage lines overexpressing the nonphosphorylatable S95A or the phosphomimicking S95D mutation. The efficiency of phagosome formation and maturation was severely reduced in SNAP-23-S95D–overexpressing cells. To examine whether phosphorylation at Ser95 affected SNAP-23 structure, we constructed intramolecular Förster resonance energy transfer (FRET) probes of SNAP-23 designed to evaluate the approximation of the N termini of the two SNARE motifs. Interestingly, a high FRET efficiency was detected on the membrane when the S95D probe was used, indicating that phosphorylation at Ser95 caused a dynamic structural shift to the closed form. Coexpression of IκB kinase (IKK) 2 enhanced the FRET efficiency of the wild-type probe on the phagosome membrane. Furthermore, the enhanced phagosomal FRET signal in interferon-γ–activated macrophages was largely dependent on IKK2, and this kinase mediated a delay in phagosome-lysosome fusion. These results suggested that SNAP-23 phosphorylation at Ser95 played an important role in the regulation of SNARE-dependent membrane fusion during FcR-mediated phagocytosis.
書誌情報 MOLECULAR BIOLOGY OF THE CELL
en : MOLECULAR BIOLOGY OF THE CELL

巻 29, 号 14, p. 1753-1762, 発行日 2018-07-15
出版者
出版者 American Society for Cell Biology
ISSN
収録物識別子タイプ ISSN
収録物識別子 10591524
DOI
関連タイプ isIdenticalTo
識別子タイプ DOI
関連識別子 10.1091/mbc.E17-08-0523
権利
権利情報 © 2018 Sakurai et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Co
情報源
関連名称 Sakurai Chiye, Itakura Makoto, Kinoshita Daiki, et al. Phosphorylation of SNAP-23 at Ser95 causes a structural alteration and negatively regulates Fc receptor-mediated phagosome formation and maturation in macrophages. MOLECULAR BIOLOGY OF THE CELL. 2018.
関連サイト
識別子タイプ URI
関連識別子 https://www.molbiolcell.org/doi/10.1091/mbc.E17-08-0523
関連名称 https://www.molbiolcell.org/doi/10.1091/mbc.E17-08-0523
著者版フラグ
出版タイプ VoR
EISSN
値 19394586
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