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Evaluation of Fluoro-Jade C Staining: Specificity and Application to Damaged Immature Neuronal Cells in the Normal and Injured Mouse Brain
https://repository.lib.tottori-u.ac.jp/records/7315
https://repository.lib.tottori-u.ac.jp/records/73157a11aaf3-f1b7-4b6e-b7f2-084b896c5483
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
n425_146.pdf (1.3 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2020-01-16 | |||||
| タイトル | ||||||
| タイトル | Evaluation of Fluoro-Jade C Staining: Specificity and Application to Damaged Immature Neuronal Cells in the Normal and Injured Mouse Brain | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | cell death | |||||
| キーワード | ||||||
| 主題 | neural stem/precursor cells | |||||
| キーワード | ||||||
| 主題 | adult neurogenesis | |||||
| キーワード | ||||||
| 主題 | embryonic brain | |||||
| キーワード | ||||||
| 主題 | glia | |||||
| キーワード | ||||||
| 主題 | mesenchymal cells | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | cell death | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | neural stem/precursor cells | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | adult neurogenesis | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | embryonic brain | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | glia | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | mesenchymal cells | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Ikenari, Takuya
× Ikenari, Takuya× Kurata, Hirofumi× Satoh, Takemasa× Hata, Yoshio× Mori, Tetsuji |
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| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Biological Regulation, School of Health Science, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Biological Regulation, School of Health Science, Faculty of Medicine, Tottori University / Division of Child Neurology, Department of Brain and Neurosciences, Faculty of Medicine, Tottori University / Department of Pediatrics, National Hospital Organization, Kumamoto Saishunso National Hospital | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Neurobiology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Neurobiology, School of Life Sciences, Faculty of Medicine, Tottori University / Division of Integrative Bioscience, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Sciences | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Biological Regulation, School of Health Science, Faculty of Medicine, Tottori University | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Fluoro-Jade C (FJC) staining is widely used for the specific detection of all degenerating mature neurons, including apoptotic, necrotic, and autophagic cells. However, whether FJC staining can detect degenerating immature neurons and neural stem/precursor cells remains unclear. In addition, some conflicting studies have shown that FJC and its ancestral dyes, Fluoro-Jade (FJ) and FJB, can label resting/activated astrocytes and microglia. In the present study, we examined the validity of FJC staining for the detection of neuronal cells in adult and embryonic mouse brains under normal and injured conditions. In the adult rodent subventricular zone (SVZ)–rostral migratory stream (RMS)–olfactory bulb (OB) system, apoptosis associated with neurogenesis occurs under normal conditions. Using this system, we detected FCJ positive (+) cells, some of which were doublecortin (DCX)(+) neuroblasts, in addition to neuronal nuclei (NeuN)(+) mature neurons. FJC negative (−) apoptotic cells expressing activated Caspase 3 were also observed, and a small number of FJC(+)/ionized calcium-binding adaptor molecule 1 (Iba1)(+) microglia and FJC(+)/glial fibrillary acidic protein (GFAP)(+) astrocytes were observed in the normal brain. Next, we analyzed embryonic brains, in which the apoptosis of neural stem/precursor cells was induced by the administration of N-ethyl-N-nitrosourea (ENU) or ethanol at embryonic day 14 or 10, respectively. In those brains, FJC(+) neural stem/precursor cells and neuroepithelial cells expressing SRY-related HMG-box 2 (Sox2) were observed. Surprisingly degenerating mesenchymal cells were also FJC(+). The present study indicates that FJC is a reliable marker for degenerating neuronal cells during all differentiation stages. However, FJC could also label degenerating non-neuronal cells under some conditions. | |||||
| 書誌情報 |
Neuroscience en : Neuroscience 巻 425, p. 146-156, 発行日 2020-01-15 |
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| 雑誌内区分 | ||||||
| 値 | Research Article | |||||
| 出版者 | ||||||
| 出版者 | Elsevier Ltd | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 03064522 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA0075489X | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.neuroscience.2019.11.029 | |||||
| 権利 | ||||||
| 権利情報 | Copyright © 2019 IBRO. Published by Elsevier Ltd. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
| 情報源 | ||||||
| 関連名称 | Ikenari, T., Kurata, H., Satoh, T., Hata, Y., & Mori, T. (2020). Evaluation of Fluoro-Jade C Staining: Specificity and Application to Damaged Immature Neuronal Cells in the Normal and Injured Mouse Brain. Neuroscience, 425, 146-156. | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.sciencedirect.com/science/article/pii/S0306452219308127 | |||||
| 関連名称 | https://www.sciencedirect.com/science/article/pii/S0306452219308127 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
