Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2018-12-14 |
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言語 |
en |
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タイトル |
Self-assembly of Ni-NTA-modified β-Annulus Peptides into Artificial Viral Capsids and Encapsulation of His-tagged Proteins |
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言語 |
eng |
資源タイプ |
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資源タイプ |
journal article |
著者 |
Matsuura, Kazunori
Nakamura, Tomohiro
Watanabe, Kenta
Noguchi, Takanori
Minamihata, Kosuke
Kamiya, Noriho
Kimizuka, Nobuo
Nakamura, Tomohiro
Watanabe, Kenta
Noguchi, Takanori
Minamihata, Kosuke
Kamiya, Noriho
Kimizuka, Nobuo
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著者所属 |
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Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University |
著者所属 |
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Department of Applied Chemistry, Graduate School of Engineering, Kyushu University |
著者所属 |
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Department of Applied Chemistry, Graduate School of Engineering, Kyushu University |
著者所属 |
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Department of Applied Chemistry, Graduate School of Engineering, Kyushu University |
著者所属 |
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Department of Applied Chemistry, Graduate School of Engineering, Kyushu University |
著者所属 |
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Department of Applied Chemistry, Graduate School of Engineering, Kyushu University/Division of Biotechnology, Center for Future Chemistry, Kyushu University |
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Department of Applied Chemistry, Graduate School of Engineering, Kyushu University/Center for Molecular Systems (CMS), Kyushu University |
著者所属(英) |
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en |
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Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University |
著者所属(英) |
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en |
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Department of Applied Chemistry, Graduate School of Engineering, Kyushu University |
著者所属(英) |
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en |
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Department of Applied Chemistry, Graduate School of Engineering, Kyushu University |
著者所属(英) |
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en |
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Department of Applied Chemistry, Graduate School of Engineering, Kyushu University |
著者所属(英) |
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en |
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Department of Applied Chemistry, Graduate School of Engineering, Kyushu University |
著者所属(英) |
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en |
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Department of Applied Chemistry, Graduate School of Engineering, Kyushu University/Division of Biotechnology, Center for Future Chemistry, Kyushu University |
著者所属(英) |
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en |
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Department of Applied Chemistry, Graduate School of Engineering, Kyushu University/Center for Molecular Systems (CMS), Kyushu University |
抄録 |
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内容記述タイプ |
Other |
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内容記述 |
β-Annulus peptide bearing Cys at the N-terminal from tomato bushy stunt virus was synthesised using a standard Fmoc-protected solid-phase method, and the petide was modified with Ni-NTA at the N-terminal. The Ni-NTA-modified β-annulus peptide self-assembled into virus-like nanocapsules of approximately 40 nm in diameter. The critical aggregation concentration of these nanocapsules in 10 mM Tris-HCl buffer (pH 7.3) at 25°C was 0.053 μM, which is 470 times lower than that of unmodified β-annulus peptides. Moreover, size exclusion chromatography of the peptide assembly indicated encapsulation of His-tagged green fluorescent protein in the Ni-NTA-modified artificial viral capsid. |
書誌情報 |
Organic & biomolecular chemistry
en : Organic & biomolecular chemistry
巻 14,
号 33,
p. 7869-7874,
発行日 2016
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出版者 |
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出版者 |
Royal Society of Chemistry |
ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
14770520 |
書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA12084375 |
DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1039/C6OB01227B |
権利 |
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権利情報 |
© The Royal Society of Chemistry 2016 |
情報源 |
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関連名称 |
This work was published by The Royal Society of Chemistry: K. Matsuura, T. Nakamura, K. Watanabe, T. Noguchi, K. Minamihata, N. Kamiyab and N. Kimizuka. Self-assembly of Ni-NTA-modified β-annulus peptides into artificial viral capsids and encapsulation |
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識別子タイプ |
URI |
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関連識別子 |
https://pubs.rsc.org/en/content/articlelanding/2016/ob/c6ob01227b |
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関連名称 |
https://pubs.rsc.org/en/content/articlelanding/2016/ob/c6ob01227b |
著者版フラグ |
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出版タイプ |
AM |