| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2020-01-08 |
| タイトル |
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タイトル |
Increased Histone H3 Phosphorylation in Neurons in Specific Brain Structures after Induction of Status Epilepticus in Mice |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
Mori, Tetsuji
Wakabayashi, Taketoshi
Ogawa, Haruyuki
Hirahara, Yukie
Koike, Taro
Yamada, Hisao
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| 著者所属（英） |
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言語 |
en |
|
値 |
Department of Anatomy and Cell Science, Kansai Medical University |
| 著者所属（英） |
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言語 |
en |
|
値 |
Department of Anatomy and Cell Science, Kansai Medical University |
| 著者所属（英） |
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言語 |
en |
|
値 |
Department of Anatomy and Cell Science, Kansai Medical University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Department of Anatomy and Cell Science, Kansai Medical University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Department of Anatomy and Cell Science, Kansai Medical University |
| 著者所属（英） |
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言語 |
en |
|
値 |
Department of Anatomy and Cell Science, Kansai Medical University |
| 抄録 |
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内容記述タイプ |
Other |
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内容記述 |
Status epilepticus (SE) induces pathological and morphological changes in the brain. Recently, it has become clear that excessive neuronal excitation, stress and drug abuse induce chromatin remodeling in neurons, thereby altering gene expression. Chromatin remodeling is a key mechanism of epigenetic gene regulation. Histone H3 phosphorylation is frequently used as a marker of chromatin remodeling and is closely related to the upregulation of mRNA transcription. In the present study, we analyzed H3 phosphorylation levels in vivo using immunohistochemistry in the brains of mice with pilocarpine-induced SE. A substantial increase in H3 phosphorylation was detected in neurons in specific brain structures. Increased H3 phosphorylation was dependent on neuronal excitation. In particular, a robust upregulation of H3 phosphorylation was detected in the caudate putamen, and there was a gradient of phosphorylated H3+ (PH3+) neurons along the medio-lateral axis. After unilateral ablation of dopaminergic neurons in the substantia nigra by injection of 6-hydroxydopamine, the distribution of PH3+ neurons changed in the caudate putamen. Moreover, our histological analysis suggested that, in addition to the well-known MSK1 (mitogen and stress-activated kinase)/H3 phosphorylation/c-fos pathway, other signaling pathways were also activated. Together, our findings suggest that a number of genes involved in the pathology of epileptogenesis are upregulated in PH3+ brain regions, and that H3 phosphorylation is a suitable indicator of strong neuronal excitation. |
| 書誌情報 |
PLOS ONE
en : PLOS ONE
巻 8,
号 10,
p. e77710,
発行日 2013-10-16
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| 出版者 |
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出版者 |
PLOS |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
19326203 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1371/journal.pone.0077710 |
| 権利 |
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権利情報 |
© 2013 Mori et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| 情報源 |
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関連名称 |
Mori T, Wakabayashi T, Ogawa H, Hirahara Y, Koike T, Yamada H (2013) Increased Histone H3 Phosphorylation in Neurons in Specific Brain Structures after Induction of Status Epilepticus in Mice. PLoS ONE 8(10): e77710. https://doi.org/10.1371/journal.pone.0 |
| 関連サイト |
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識別子タイプ |
URI |
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関連識別子 |
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0077710 |
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関連名称 |
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0077710 |
| 著者版フラグ |
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出版タイプ |
VoR |
po8(10)_e77710.PDF (4.2 MB)
