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Cloning of a Putative Vesicle Transport-related Protein, RA410, from Cultured Rat Astrocytes and Its Expression in Ischemic Rat Brain
https://repository.lib.tottori-u.ac.jp/records/7381
https://repository.lib.tottori-u.ac.jp/records/73815887c061-74c3-49b9-aaf8-8752f24c27b8
| 名前 / ファイル | ライセンス | アクション |
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jbc272(26)_16438.pdf (647.9 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2019-12-24 | |||||
| タイトル | ||||||
| タイトル | Cloning of a Putative Vesicle Transport-related Protein, RA410, from Cultured Rat Astrocytes and Its Expression in Ischemic Rat Brain | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Matsuo, Noriyuki
× Matsuo, Noriyuki× Ogawa, Satoshi× Takagi, Tsutomu× Wanaka, Akio× Mori, Tetsuji× Matsuyama, Tomohiro× J., Pinsky David× M., Stern David× Tohyama, Masaya |
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| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Anatomy and Neuroscience, Osaka University Medical School / Dainippon Pharmaceutical Company Limited | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Anatomy and Neuroscience, Osaka University Medical School | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Molecular Neurobiology (Tanabe), Osaka University Medical School | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Cell Science, Institute of Biomedical Sciences, Fukushima Medical College | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Cell Science, Institute of Biomedical Sciences, Fukushima Medical College | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Anatomy and Neuroscience, Osaka University Medical School | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Physiology and Cellular Biophysics and Surgery, College of Physicians and Surgeons, Columbia University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Physiology and Cellular Biophysics and Surgery, College of Physicians and Surgeons, Columbia University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Anatomy and Neuroscience, Osaka University Medical School | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | To elucidate the role of astrocytes in the stress response of the central nervous system to ischemia, early gene expression was evaluated in cultured rat astrocytes subjected to hypoxia/reoxygenation. Using differential display, a novel putative vesicle transport-related factor (RA410) was cloned from reoxygenated astrocytes. Analysis of the deduced amino acid sequence showed RA410 to be composed of domains common to vesicle transport-related proteins of the Sec1/Unc18 family, including Sly1p and Sec1p (yeast), Rop (Drosophila), Unc18 (Caenorhabditis elegans), and Munc18 (mammalian), suggesting its possible role in vesicular transport. Northern analysis of normal rat tissues showed the highest expression of RA410 transcripts in testis. When astrocyte cultures were subjected to a period of hypoxia followed by reoxygenation, induction of RA410 mRNA was observed within 15 min of reoxygenation, reaching a maximum by 60 min. At the start of reoxygenation, the addition of diphenyl iodonium, an NADPH oxidase inhibitor, blocked in parallel astrocyte generation of reactive oxygen intermediates and expression of RA410 message. In contrast, cycloheximide did not affect RA410 mRNA levels, indicating that RA410 is an immediate-early gene in the setting of reoxygenation. Using polyclonal antibody raised against an RA410-derived synthetic peptide, Western blotting of lysates from reoxygenated astrocytes displayed an immunoreactive band of ≈70 kDa, the expression of which followed induction of the mRNA. Fractionation of astrocyte lysates on sucrose gradients showed RA410 antigen to be predominantly in the plasma membrane. Immunoelectron microscopic analysis demonstrated RA410 in large vesicles associated with the Golgi, but not in the Golgi apparatus itself, consistent with its participation in post-Golgi transport. Consistent with thesein vitro data, RA410 expression was observed in rat brain astrocytes following transient occlusion of the middle cerebral artery. These data provide insight into a new protein (RA410) that participates in the ischemia-related stress response in astrocytes. | |||||
| 書誌情報 |
The journal of biological chemistry en : The journal of biological chemistry 巻 272, 号 26, p. 16438-16444, 発行日 1997-06-27 |
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| 出版者 | ||||||
| 出版者 | The American Society for Biochemistry and Molecular Biology | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1083351X | |||||
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| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA1202441X | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1074/jbc.272.26.16438 | |||||
| 権利 | ||||||
| 権利情報 | © the American Society for Biochemistry and Molecular Biology | |||||
| 情報源 | ||||||
| 関連名称 | This research was originally published in the Journal of Biological Chemistry. Matsuo, Noriyuki, et al. Cloning of a putative vesicle transport-related protein, RA410, from cultured rat astrocytes and its expression in ischemic rat brain. J. Biol. Chem. 1 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.jbc.org/content/272/26/16438 | |||||
| 関連名称 | http://www.jbc.org/content/272/26/16438 | |||||
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| 出版タイプ | VoR | |||||
