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Rapid Multiplex RT-PCR for Influenza A and B by Genesoc®, a Microfluidic PCR System
https://repository.lib.tottori-u.ac.jp/records/7693
https://repository.lib.tottori-u.ac.jp/records/7693447d5885-3aa9-4013-823a-46ed9db475fc
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
yam66(2)_223.pdf (2.8 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2023-07-18 | |||||
| タイトル | ||||||
| タイトル | Rapid Multiplex RT-PCR for Influenza A and B by Genesoc®, a Microfluidic PCR System | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | antigen detection test | |||||
| キーワード | ||||||
| 主題 | GeneSoC® | |||||
| キーワード | ||||||
| 主題 | influenza A | |||||
| キーワード | ||||||
| 主題 | influenza B | |||||
| キーワード | ||||||
| 主題 | realtime-PCR | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | antigen detection test | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | GeneSoC® | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | influenza A | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | influenza B | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | realtime-PCR | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
高田, 美也子
× 高田, 美也子× 中本, 成紀× 北浦, 剛× 岡田, 健作× 常城, 朱乃× 山崎, 章× 景山, 誠二× 鰤岡, 直人× 千酌, 浩樹× Tsuneki-tokunaga, Akeno |
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| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Pathobiological Science and Technology, Graduate School of Medical Science, School of Health Science, Facultyof Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Infectious Diseases, School of Medicine, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Infectious Diseases, School of Medicine, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Infectious Diseases, School of Medicine, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Virology, Department of Microbiology and Immunology, School of Medicine, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Respiratory medicine and Rheumatology, Department of Multidisciplinary Internal Medicine, School of Medicine, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Virology, Department of Microbiology and Immunology, School of Medicine, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Pathobiological Science and Technology, Graduate School of Medical Science, School of Health Science, Facultyof Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Infectious Diseases, School of Medicine, Faculty of Medicine, Tottori UniversityDepartment of Pathobiological Science and Technology, Graduate School of Medical Science, School of Health Science, Facultyof Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Infectious Diseases, School of Medicine, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Infectious Diseases, School of Medicine, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Infectious Diseases, School of Medicine, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Virology, Department of Microbiology and Immunology, School of Medicine, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Respiratory medicine and Rheumatology, Department of Multidisciplinary Internal Medicine, School of Medicine, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Virology, Department of Microbiology and Immunology, School of Medicine, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Pathobiological Science and Technology, Graduate School of Medical Science, School of Health Science, Facultyof Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Infectious Diseases, School of Medicine, Faculty of Medicine, Tottori University | |||||
| 著者ID | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 03617 | |||||
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| 内容記述タイプ | Other | |||||
| 内容記述 | 04846 | |||||
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| 内容記述 | 01594 | |||||
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| 内容記述 | 02849 | |||||
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| 内容記述 | 03988 | |||||
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| 内容記述 | 04358 | |||||
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| 内容記述タイプ | Other | |||||
| 内容記述 | 01213 | |||||
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| 内容記述タイプ | Other | |||||
| 内容記述 | 00196 | |||||
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| 内容記述タイプ | Other | |||||
| 内容記述 | 00212 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Background: Rapid antigen tests are widely used to diagnose influenza. However, despite their simplicity and short turnover time, the sensitivity of these tests is relatively low, and molecular tests with greater sensitivity are being sought. In this study, we developed and clinically evaluated a protocol for the rapid multiplex testing of influenza A and B, using a rapid real-time PCR system, GeneSoC®, that is based on microfluidic thermal cycling technology. Methods: The specificity of the developed assay was validated using cultured viral strains of influenza A/B, human metapneumovirus, and respiratory syncytial virus. Analytical sensitivity was evaluated using serially diluted RNA synthesized via in vitro transcription and nasopharyngeal swab samples collected from consecutive patients seeking medical attention for a combination of upper respiratory and general symptoms. Cross-validation of GeneSoC® based on comparisons with conventional real-time RT-PCR and rapid antigen tests was performed by parallel testing of influenza-positive clinical specimens. Results: The GeneSoC® assay detected the target sequences of influenza A and B at minimum concentrations of 38 and 65 copies/µL in reaction, respectively. For the analysis of clinical specimens, the positive, negative, and overall agreement between GeneSoC® RT-PCR and a conventional real-time RT-PCR was in all cases 100%, whereas for the comparison between GeneSoC® RT-PCR and the rapid antigen test, the agreements for positive, negative, and overall findings were 100%, 90.9%, and 95.7%, respectively. The mean time for completing GeneSoC® RT-PCR was 16 min 29 s (95% confidence interval, 16 min 18 s to 16 min 39 s). Conclusion: The microfluidic real-time PCR system, GeneSoC®, has an analytical performance comparable to that of conventional real-time RT-PCR with rapid turnover time, and represents a promising alternative to rapid antigen tests for diagnosing influenza A and B. | |||||
| 書誌情報 |
Yonago Acta Medica en : Yonago Acta Medica 巻 66, 号 2, p. 223-231, 発行日 2023-05-25 |
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| 出版者 | ||||||
| 出版者 | Tottori University Medical Press | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 05135710 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00892882 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.33160/yam.2023.05.007 | |||||
| 権利 | ||||||
| 権利情報 | (C) 2023 Tottori University Medical Press. | |||||
| 情報源 | ||||||
| 関連名称 | Yonago Acta Medica. 2023, 66(2), 223-231. doi10.33160/yam.2023.05.007 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.lib.tottori-u.ac.jp/yam/yam/yam66-2/66-2contents.html | |||||
| 関連名称 | https://www.lib.tottori-u.ac.jp/yam/yam/yam66-2/66-2contents.html | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.33160/yam.2023.05.007 | |||||
| 関連名称 | https://doi.org/10.33160/yam.2023.05.007 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| EISSN | ||||||
| 値 | 13468049 | |||||
