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Dynamic assembly and interaction of glycosphingolipids in cholesterol-containing model membranes
https://repository.lib.tottori-u.ac.jp/records/2001320
https://repository.lib.tottori-u.ac.jp/records/20013200bee6011-f518-4e4b-b851-024766aa7d35
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
pac95(9)_971.pdf
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(C) 2023 IUPAC & De Gruyter
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||
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| 公開日 | 2024-02-21 | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | Dynamic assembly and interaction of glycosphingolipids in cholesterol-containing model membranes | |||||||||||||||
| 言語 | en | |||||||||||||||
| 言語 | ||||||||||||||||
| 言語 | eng | |||||||||||||||
| キーワード | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 主題 | Bilayer membranes | |||||||||||||||
| キーワード | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 主題 | fluorescence spectroscopy | |||||||||||||||
| キーワード | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 主題 | glycosphingolipids | |||||||||||||||
| キーワード | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 主題 | interaction | |||||||||||||||
| キーワード | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 主題 | ICS-30 | |||||||||||||||
| キーワード | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 主題 | NMR | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||
| アクセス権 | ||||||||||||||||
| アクセス権 | open access | |||||||||||||||
| 著者 |
花島,慎弥
× 花島,慎弥
研究者総覧鳥取大学
100002461
× Nakano,Mikito
× Murata,Michio
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| 著者所属（英） | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 値 | Graduate School of Science, Osaka University / Graduate School of Engineering, Tottori University | |||||||||||||||
| 著者所属（英） | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 値 | Graduate School of Science, Osaka University | |||||||||||||||
| 著者所属（英） | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 値 | Michio Murata, Graduate School of Science, Osaka University / ForeFront Research Center, Osaka University | |||||||||||||||
| 抄録 | ||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||
| 内容記述 | Glycosphingolipids (GSLs) in the mammalian plasma membrane are essential for various biological events that occur on cell membranes by forming glycolipid-rich membrane domains, such as lipid rafts. Lactosylceramide (LacCer) forms a highly ordered phase in model and biological membranes. However, the details of this domain remain unclear. We examined the dynamic assembly of LacCer in cholesterol-containing phase-separated membranes by fluorescence imaging and solid-state NMR. Solid-state 2H NMR of the deuterated LacCer probes in the membranes indicated that the potent LacCer–LacCer interaction significantly reduced the incorporation of cholesterol in the LacCer domains. Moreover, increasing the amount of cholesterol induced the formation of nanometer-scale LacCer domains, which can be biologically relevant. The lateral interaction of ganglioside GM3 with the epidermal growth factor receptor (EGFR) transmembrane segment was examined using fluorescence spectroscopy under membrane conditions. Lateral FRET between the NBD fluorophore on the EGFR transmembrane segment and the ATTO594 fluorophore on the GM3 head group suggested that GM3 interacts with the transmembrane segment and partially inhibits the EGFR dimerization by stabilizing the peptide monomer. More precise investigations of GSL–GSL and GSL-protein interactions occurring in membrane environments can lead to the elucidation of the cellular functions of GSL from structural points of view. | |||||||||||||||
| 言語 | en | |||||||||||||||
| 書誌情報 |
en : Pure and Applied Chemistry 巻 95, 号 9, p. 971-977, ページ数 7, 発行日 2023-09-26 |
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| 出版者 | ||||||||||||||||
| 出版者 | IUPAC & De Gruyter | |||||||||||||||
| 言語 | en | |||||||||||||||
| ISSN | ||||||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||||||
| 収録物識別子 | 00334545 | |||||||||||||||
| 権利 | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 権利情報 | (C) 2023 IUPAC & De Gruyter | |||||||||||||||
| 情報源 | ||||||||||||||||
| 関連名称 | Hanashima, Shinya, Nakano, Mikito and Murata, Michio. "Dynamic assembly and interaction of glycosphingolipids in cholesterol-containing model membranes" Pure and Applied Chemistry, vol. 95, no. 9, 2023, pp. 971-977. https://doi.org/10.1515/pac-2023-0204 | |||||||||||||||
| 関連サイト | ||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||
| 関連識別子 | https://doi.org/10.1515/pac-2023-0204 | |||||||||||||||
| 関連名称 | https://doi.org/10.1515/pac-2023-0204 | |||||||||||||||
| 著者版フラグ | ||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||
| Web of Science Key ut | ||||||||||||||||
| 値 | WOS:000980113000001 | |||||||||||||||
