| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2025-01-20 |
| タイトル |
|
|
タイトル |
In Vitro Efficacy of Photodynamic Antimicrobial Chemotherapy with TONS504 Using Blue Light Against Pseudomonas aeruginosa |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
言語 |
en |
|
主題 |
blue light |
| キーワード |
|
|
言語 |
en |
|
主題 |
EDTA |
| キーワード |
|
|
言語 |
en |
|
主題 |
photochemotherapy |
| キーワード |
|
|
言語 |
en |
|
主題 |
Pseudomonas aeruginosa |
| キーワード |
|
|
言語 |
en |
|
主題 |
TONS504 |
| 資源タイプ |
|
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
open access |
| 著者 |
Furujo, Tomoya
Ito, Norihiko
原田, 和記
Sakata, Isao
大崎, 智弘
|
| 抄録 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Background Pseudomonas aeruginosa is an important causative agent of bacterial keratitis. This study investigates the antibacterial effect of photodynamic antimicrobial chemotherapy (PACT) with the chlorin derivative TONS504 (TONS504-PACT) and a blue light-emitting diode (LED) on P. aeruginosa in vitro. It also explores the synergistic effects of combining TONS504-PACT with EDTA, and compares the antibacterial effects of TONS504-PACT using blue light with those of previously reported red light. Methods To evaluate the antibacterial effects of TONS504-PACT using blue light on P. aeruginosa, PACT with TONS504 (0–100 mg/L) was applied using a 405 nm LED at 0–30 J/cm2 to P. aeruginosa (107–8 CFU/mL). The antibacterial effects were assessed by calculating the survival fraction. Subsequently, to compare the effects of TONS504-PACT using blue light with those of using red light, PACT with TONS504 (10 or 100 mg/L) was conducted using a 405 or 660 nm LED at 30 J/cm2 and evaluated in the same manner. Finally, to investigate the synergistic antibacterial effects of TONS504-PACT with EDTA, PACT with TONS504 (0–100 mg/L) was applied using a 405 nm LED at 5 J/cm2, both with and without EDTA (0.015 M), and its antibacterial effects were similarly assessed. Results TONS504-PACT using blue light decreased the survival of P. aeruginosa, depending on the light intensity. The greatest antibacterial effect was observed at 10 mg/L and 30 J/cm2, showing a survival fraction of 3.5 × 10-5. TONS504-PACT demonstrated a significantly greater antibacterial effect using blue light than that demonstrated using red light at 10 mg/L, though not at 100 mg/L. The combination of TONS504-PACT using blue light with EDTA significantly enhanced antibacterial effects at all concentrations. Conclusion These findings confirm the effectiveness of TONS504-PACT using blue light against P. aeruginosa and suggest its potential as a treatment for bacterial keratitis. |
|
言語 |
en |
| 書誌情報 |
en : Yonago Acta Medica
巻 67,
号 4,
p. 284-292,
ページ数 9,
発行日 2024-11-27
|
| 出版者 |
|
|
出版者 |
Tottori University Medical Press |
|
言語 |
en |
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
05135710 |
| ISSN |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
13468049 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00892882 |
| 権利 |
|
|
権利情報 |
(C)2024 Tottori University Medical Press |
|
言語 |
en |
| 情報源 |
|
|
|
言語 |
en |
|
|
関連名称 |
Yonago Acta Medica. 2024, 67(4), 284-292. |
| 関連サイト |
|
|
|
識別子タイプ |
URI |
|
|
関連識別子 |
https://www.lib.tottori-u.ac.jp/yam/yam/yam67-4/67-4contents.html |
|
|
関連名称 |
https://www.lib.tottori-u.ac.jp/yam/yam/yam67-4/67-4contents.html |
| 関連サイト |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.33160/yam.2024.11.001 |
|
|
関連名称 |
https://doi.org/10.33160/yam.2024.11.001 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
yam67(4)_284.pdf (874 KB)
