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Effect of glucosylation for the vertical movement of cholesterol in bilayer membranes
https://repository.lib.tottori-u.ac.jp/records/2001868
https://repository.lib.tottori-u.ac.jp/records/2001868f76c022e-9d8b-48c4-b2aa-87d7d76b0443
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
bbamem1867(5-6)_184422.pdf (1.1 MB)
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| Item type | デフォルトアイテムタイプ（フル）(1) | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2025-05-28 | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | Effect of glucosylation for the vertical movement of cholesterol in bilayer membranes | |||||||||||||||
| 言語 | en | |||||||||||||||
| 作成者 |
花島,慎弥
× 花島,慎弥
研究者総覧鳥取大学
100002461_ja.html
× Asahina,Takafumi
× Malabed,Raymond
× Sasaki,Katsuaki
× Murata,Michio |
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| 主題 | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | Cholesterol | |||||||||||||||
| 主題 | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | Steryl glucoside | |||||||||||||||
| 主題 | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | Glucosylation | |||||||||||||||
| 主題 | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | Bilayer membrane | |||||||||||||||
| 主題 | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 主題Scheme | Other | |||||||||||||||
| 主題 | Flip-flop | |||||||||||||||
| 内容記述 | ||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||
| 内容記述 | Cholesterol (Chol) in mammalian cell membranes facilitates the assembly of dynamic membrane domains that are involved in vital biological processes through lateral and transbilayer movements in the membranes. In the cell membranes, Chol undergoes glucose transglycosylation to produce cholesteryl-β-d-glucoside (ChoGlc). ChoGlc is involved in neurodegenerative diseases and accumulates in lysosomal storage disorders. However, the effects of glucosylation on membrane properties of Chol remain unclear. We investigated the membrane interaction of ChoGlc and its subsequent translocation between leaflets using fluorescent probes, such as 4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) and the newly synthesized 7-nitro-2,1,3-benzoxadiazole-labeled ChoGlc (NBD-ChoGlc) in dioleoylphosphatidylcholine (DOPC) membranes. The fluorescence of TMA-DPH, which selectively reported the order of the outer leaflet of the bilayer, indicated that ChoGlc added to the external solution, was mostly incorporated into the membranes and increased the DOPC membrane ordering. Furthermore, the anisotropy values reached a level similar to that of the ChoGlc-preloaded symmetric vesicle within approximately 5 min owing to the rapid distribution of ChoGlc in both leaflets. This was further confirmed by the selective fluorescence quenching of NBD-ChoGlc in the outer leaflet through irreversible quenching by dithionite. The similarity of the fluorescence decay curves of NBD-ChoGlc and NBD-Chol indicated that the glucosylation had little impact on the flip-flops of Chol in the DOPC bilayers. Our data demonstrates that some of the important membrane properties of Chol, such as fast flip-flop between leaflets and increased membrane order, were mostly maintained in ChoGlc despite hydrophilic glucose modification. | |||||||||||||||
| 言語 | en | |||||||||||||||
| 出版者 | ||||||||||||||||
| 出版者 | Elsevier | |||||||||||||||
| 言語 | en | |||||||||||||||
| 日付 | ||||||||||||||||
| 日付 | 2025-05-08 | |||||||||||||||
| 日付タイプ | Issued | |||||||||||||||
| 言語 | ||||||||||||||||
| 言語 | eng | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
| 資源タイプ | journal article | |||||||||||||||
| 出版タイプ | ||||||||||||||||
| 出版タイプ | AM | |||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||
| 関連情報 | ||||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||
| 関連識別子 | https://doi.org/10.1016/j.bbamem.2025.184422 | |||||||||||||||
| 収録物識別子 | ||||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||||
| 収録物識別子 | 18792642 | |||||||||||||||
| 書誌情報 |
en : Biochimica et Biophysica Acta (BBA) - Biomembranes 巻 1867, 号 5-6, p. 184422, 発行日 2025-05-08 |
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| 出版者情報 | ||||||||||||||||
| 出版者名 | Elsevier | |||||||||||||||
| 言語 | en | |||||||||||||||
| アクセス権 | ||||||||||||||||
| アクセス権 | embargoed access | |||||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_f1cf | |||||||||||||||
| 権利情報 | ||||||||||||||||
| 言語 | en | |||||||||||||||
| 権利情報 | Copyright © 2025 Elsevier B.V. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||||||||||||
