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Comparison of Characterization in Two-Dimensional and Three-Dimensional Canine Mammary Gland Tumor Cell Models
https://repository.lib.tottori-u.ac.jp/records/4604
https://repository.lib.tottori-u.ac.jp/records/460468a65541-8b8e-4ea3-ad6f-a6ad52bc17d3
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
yam66(1)_7.pdf (6.6 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2023-02-28 | |||||
| タイトル | ||||||
| タイトル | Comparison of Characterization in Two-Dimensional and Three-Dimensional Canine Mammary Gland Tumor Cell Models | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | canine | |||||
| キーワード | ||||||
| 主題 | mammary gland tumor | |||||
| キーワード | ||||||
| 主題 | microRNA | |||||
| キーワード | ||||||
| 主題 | three-dimensional | |||||
| キーワード | ||||||
| 主題 | two-dimensional | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | canine | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | mammary gland tumor | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | microRNA | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | three-dimensional | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | two-dimensional | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
尾崎, 知博
× 尾崎, 知博× 寸田, 祐嗣× 割田, 克彦× 岡本, 芳晴 |
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| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Background: Canine mammary gland tumors can be used as predictive models for human breast cancer. There are several types of microRNAs common in human breast cancer and canine mammary gland tumors. The functions of microRNAs in canine mammary gland tumors are not well understood. Methods: We compared the characterization of microRNA expression in two-dimensional and three-dimensional canine mammary gland tumor cell models. We evaluated the differences between two- and three-dimensional cultured canine mammary gland tumor SNP cells by assessing microRNA expression levels, morphology, drug sensitivity, and hypoxia. Results: The expression of microRNA-210 in the three-dimensional-SNP cells was 10.19 times higher than that in the two-dimensional-SNP cells. The intracellular concentrations of doxorubicin in the two- and three-dimensional-SNP cells were 0.330 ± 0.013 and 0.290 ± 0.048 nM/mg protein, respectively. The IC50 values of doxorubicin for the two- and three-dimensional-SNP cells were 5.2 and 1.6 μM, respectively. Fluorescence of the hypoxia probe, LOX-1, was observed inside the sphere of three-dimensional-SNP cells without echinomycin but not in two-dimensional-SNP cells. The three-dimensional-SNP cells treated with echinomycin showed weak LOX-1 fluorescence. Conclusion: The present study showed a clear difference in microRNA expression levels in cells cultured in a two-dimensional adherent versus a three-dimensional spheroid model. | |||||
| 書誌情報 |
Yonago Acta Medica en : Yonago Acta Medica 巻 66, 号 1, p. 7-18, 発行日 2023-02-20 |
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| 出版者 | ||||||
| 出版者 | Tottori University Medical Press | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 05135710 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00892882 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.33160/yam.2023.02.002 | |||||
| 権利 | ||||||
| 権利情報 | (C) 2023 Tottori University Medical Press. | |||||
| 情報源 | ||||||
| 関連名称 | Yonago Acta Medica. 2023, 66(1), 7-18. doi:10.33160/yam.2023.02.002 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.lib.tottori-u.ac.jp/yam/yam/yam66-1/66-1contents.html | |||||
| 関連名称 | https://www.lib.tottori-u.ac.jp/yam/yam/yam66-1/66-1contents.html | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.33160/yam.2023.02.002 | |||||
| 関連名称 | https://doi.org/10.33160/yam.2023.02.002 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| EISSN | ||||||
| 値 | 13468049 | |||||
