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Pathological Comparison of TDP-43 Between Motor Neurons and Interneurons Expressed by a Tetracycline Repressor System on the Mouse Artificial Chromosome
https://repository.lib.tottori-u.ac.jp/records/4606
https://repository.lib.tottori-u.ac.jp/records/460676ed80b1-3455-40c8-b304-f38042bc104d
名前 / ファイル | ライセンス | アクション |
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(C) 2023 Tottori University Medical Press.
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2023-02-28 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Pathological Comparison of TDP-43 Between Motor Neurons and Interneurons Expressed by a Tetracycline Repressor System on the Mouse Artificial Chromosome | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | doxycycline | |||||
キーワード | ||||||
主題 | interneuron | |||||
キーワード | ||||||
主題 | motor neuron | |||||
キーワード | ||||||
主題 | mouse artificial chromosome | |||||
キーワード | ||||||
主題 | TAR-DNA binding protein of 43 kDa | |||||
キーワード | ||||||
言語 | en | |||||
主題 | doxycycline | |||||
キーワード | ||||||
言語 | en | |||||
主題 | interneuron | |||||
キーワード | ||||||
言語 | en | |||||
主題 | motor neuron | |||||
キーワード | ||||||
言語 | en | |||||
主題 | mouse artificial chromosome | |||||
キーワード | ||||||
言語 | en | |||||
主題 | TAR-DNA binding protein of 43 kDa | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
著者 |
香月, 康宏
× 香月, 康宏× 平塚, 正治× Togai, Shota× Hamamichi, Shusei |
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著者所属(英) | ||||||
en | ||||||
Department of Chromosome Biomedical Engineering, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Tottori University | ||||||
著者所属(英) | ||||||
en | ||||||
Chromosome Engineering Research Center, Tottori University | ||||||
著者所属(英) | ||||||
en | ||||||
Department of Chromosome Biomedical Engineering, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Tottori University / Chromosome Engineering Research Center, Tottori University / Department of Chromosome Biomedical Engineering, School of Life Sciences, Faculty of Medicine, Tottori University / Chromosome Engineering Research Group, The Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences | ||||||
著者所属(英) | ||||||
en | ||||||
Chromosome Engineering Research Center, Tottori University / Department of Chromosome Biomedical Engineering, School of Life Sciences, Faculty of Medicine, Tottori University | ||||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Background: Cytoplasmic mislocalization of TAR-DNA binding protein of 43 kDa (TDP-43) is a major hallmark of amyotrophic lateral sclerosis (ALS). TDP-43 aggregation is detected in the cortical and spinal motor neurons in most ALS cases; however, pathological mechanism of this mislocalized TDP-43 remains unknown. Methods: We generated a tetracycline-inducible TDP-43 A315T system on a mouse artificial chromosome (MAC) vector to avoid transgene-insertional mutagenesis, established a mouse embryonic stem (ES) cell line holding this MAC vector system, and investigated whether overexpressed exogenous TDP-43 A315T was mislocalized in the cytoplasm of the ES cell-derived neurons and triggered the neurotoxic effects on these cells. Results: Inducible TDP-43 A315T system was successfully loaded onto the MAC and introduced into the mouse ES cells. These ES cells could differentiate into motor neurons and interneurons. Overexpression of TDP-43 A315T by addition of doxycycline in both neurons resulted in mislocalization to cytoplasm. Mislocalized TDP-43 caused cell death of motor neurons, but not interneurons. Conclusion: Vulnerability to cytoplasmic mislocalized TDP-43 is selective on neuronal types, whereas mislocalization of overexpressed TDP-43 occurs in even insusceptible neurons. This inducible gene expression system using MAC remains useful for providing critical insights into appearance of TDP-43 pathology. | |||||
書誌情報 |
Yonago Acta Medica en : Yonago Acta Medica 巻 66, 号 1, p. 24-35, 発行日 2023-02-20 |
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出版者 | ||||||
出版者 | Tottori University Medical Press | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 05135710 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00892882 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.33160/yam.2023.02.004 | |||||
権利 | ||||||
権利情報 | (C) 2023 Tottori University Medical Press. | |||||
情報源 | ||||||
関連名称 | Yonago Acta Medica. 2023, 66(1), 24-35. doi:10.33160/yam.2023.02.004 | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.lib.tottori-u.ac.jp/yam/yam/yam66-1/66-1contents.html | |||||
関連名称 | https://www.lib.tottori-u.ac.jp/yam/yam/yam66-1/66-1contents.html | |||||
関連サイト | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.33160/yam.2023.02.004 | |||||
関連名称 | https://doi.org/10.33160/yam.2023.02.004 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
EISSN | ||||||
13468049 |