| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2023-02-28 |
| タイトル |
|
|
タイトル |
Pathological Comparison of TDP-43 Between Motor Neurons and Interneurons Expressed by a Tetracycline Repressor System on the Mouse Artificial Chromosome |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
doxycycline |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
interneuron |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
motor neuron |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
mouse artificial chromosome |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
TAR-DNA binding protein of 43 kDa |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
doxycycline |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
interneuron |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
motor neuron |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
mouse artificial chromosome |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
TAR-DNA binding protein of 43 kDa |
| 資源タイプ |
|
|
資源タイプ |
journal article |
| 著者 |
香月, 康宏
平塚, 正治
Togai, Shota
Hamamichi, Shusei
|
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Department of Chromosome Biomedical Engineering, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Chromosome Engineering Research Center, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Department of Chromosome Biomedical Engineering, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Tottori University / Chromosome Engineering Research Center, Tottori University / Department of Chromosome Biomedical Engineering, School of Life Sciences, Faculty of Medicine, Tottori University / Chromosome Engineering Research Group, The Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Chromosome Engineering Research Center, Tottori University / Department of Chromosome Biomedical Engineering, School of Life Sciences, Faculty of Medicine, Tottori University |
| 抄録 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Background: Cytoplasmic mislocalization of TAR-DNA binding protein of 43 kDa (TDP-43) is a major hallmark of amyotrophic lateral sclerosis (ALS). TDP-43 aggregation is detected in the cortical and spinal motor neurons in most ALS cases; however, pathological mechanism of this mislocalized TDP-43 remains unknown. Methods: We generated a tetracycline-inducible TDP-43 A315T system on a mouse artificial chromosome (MAC) vector to avoid transgene-insertional mutagenesis, established a mouse embryonic stem (ES) cell line holding this MAC vector system, and investigated whether overexpressed exogenous TDP-43 A315T was mislocalized in the cytoplasm of the ES cell-derived neurons and triggered the neurotoxic effects on these cells. Results: Inducible TDP-43 A315T system was successfully loaded onto the MAC and introduced into the mouse ES cells. These ES cells could differentiate into motor neurons and interneurons. Overexpression of TDP-43 A315T by addition of doxycycline in both neurons resulted in mislocalization to cytoplasm. Mislocalized TDP-43 caused cell death of motor neurons, but not interneurons. Conclusion: Vulnerability to cytoplasmic mislocalized TDP-43 is selective on neuronal types, whereas mislocalization of overexpressed TDP-43 occurs in even insusceptible neurons. This inducible gene expression system using MAC remains useful for providing critical insights into appearance of TDP-43 pathology. |
| 書誌情報 |
Yonago Acta Medica
en : Yonago Acta Medica
巻 66,
号 1,
p. 24-35,
発行日 2023-02-20
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| 出版者 |
|
|
出版者 |
Tottori University Medical Press |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
05135710 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00892882 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.33160/yam.2023.02.004 |
| 権利 |
|
|
権利情報 |
(C) 2023 Tottori University Medical Press. |
| 情報源 |
|
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|
関連名称 |
Yonago Acta Medica. 2023, 66(1), 24-35. doi:10.33160/yam.2023.02.004 |
| 関連サイト |
|
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|
識別子タイプ |
URI |
|
|
関連識別子 |
https://www.lib.tottori-u.ac.jp/yam/yam/yam66-1/66-1contents.html |
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|
関連名称 |
https://www.lib.tottori-u.ac.jp/yam/yam/yam66-1/66-1contents.html |
| 関連サイト |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.33160/yam.2023.02.004 |
|
|
関連名称 |
https://doi.org/10.33160/yam.2023.02.004 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
| EISSN |
|
|
値 |
13468049 |
yam66(1)_24.pdf (6.2 MB)
