{"_buckets": {"deposit": "90881a9a-8534-4122-9773-dee87865f8d7"}, "_deposit": {"created_by": 1, "id": "4606", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "4606"}, "status": "published"}, "_oai": {"id": "oai:repository.lib.tottori-u.ac.jp:00004606", "sets": ["9", "12", "976"]}, "author_link": ["1469", "16485", "16486", "743"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2023-02-20", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "35", "bibliographicPageStart": "24", "bibliographicVolumeNumber": "66", "bibliographic_titles": [{"bibliographic_title": "Yonago Acta Medica"}, {"bibliographic_title": "Yonago Acta Medica", "bibliographic_titleLang": "en"}]}]}, "item_10001_checkbox_27": {"attribute_name": "部局名", "attribute_value_mlt": [{"subitem_checkbox_item": ["i-gakubu"]}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Background: Cytoplasmic mislocalization of TAR-DNA binding protein of 43 kDa (TDP-43) is a major hallmark of amyotrophic lateral sclerosis (ALS). TDP-43 aggregation is detected in the cortical and spinal motor neurons in most ALS cases; however, pathological mechanism of this mislocalized TDP-43 remains unknown. Methods: We generated a tetracycline-inducible TDP-43 A315T system on a mouse artificial chromosome (MAC) vector to avoid transgene-insertional mutagenesis, established a mouse embryonic stem (ES) cell line holding this MAC vector system, and investigated whether overexpressed exogenous TDP-43 A315T was mislocalized in the cytoplasm of the ES cell-derived neurons and triggered the neurotoxic effects on these cells. Results: Inducible TDP-43 A315T system was successfully loaded onto the MAC and introduced into the mouse ES cells. These ES cells could differentiate into motor neurons and interneurons. Overexpression of TDP-43 A315T by addition of doxycycline in both neurons resulted in mislocalization to cytoplasm. Mislocalized TDP-43 caused cell death of motor neurons, but not interneurons. Conclusion: Vulnerability to cytoplasmic mislocalized TDP-43 is selective on neuronal types, whereas mislocalization of overexpressed TDP-43 occurs in even insusceptible neurons. This inducible gene expression system using MAC remains useful for providing critical insights into appearance of TDP-43 pathology.", "subitem_description_type": "Abstract"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Tottori University Medical Press"}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.33160/yam.2023.02.004", "subitem_relation_type_select": "DOI"}}]}, "item_10001_relation_16": {"attribute_name": "情報源", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "Yonago Acta Medica. 2023, 66(1), 24-35. doi:10.33160/yam.2023.02.004"}]}]}, "item_10001_relation_17": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "https://www.lib.tottori-u.ac.jp/yam/yam/yam66-1/66-1contents.html"}], 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Chromosome Biomedical Engineering, School of Life Sciences, Faculty of Medicine, Tottori University / Chromosome Engineering Research Group, The Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences"}, {"subitem_text_language": "en", "subitem_text_value": "Chromosome Engineering Research Center, Tottori University / Department of Chromosome Biomedical Engineering, School of Life Sciences, Faculty of Medicine, Tottori University"}]}, "item_10001_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "香月, 康宏"}, {"creatorName": "カズキ, ヤスヒロ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Kazuki, Yasuhiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1469", 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"nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hamamichi, Shusei", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "16486", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2023-03-15"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "yam66(1)_24.pdf", "filesize": [{"value": "6.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "(C) 2023 Tottori University Medical Press.", "licensetype": "license_free", "mimetype": "application/pdf", "size": 6200000.0, "url": {"label": "yam66(1)_24.pdf", "url": "https://repository.lib.tottori-u.ac.jp/record/4606/files/yam66(1)_24.pdf"}, "version_id": "14cb2aaa-c728-4361-958b-4e924f5d9705"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": 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"言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Pathological Comparison of TDP-43 Between Motor Neurons and Interneurons Expressed by a Tetracycline Repressor System on the Mouse Artificial Chromosome", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Pathological Comparison of TDP-43 Between Motor Neurons and Interneurons Expressed by a Tetracycline Repressor System on the Mouse Artificial Chromosome", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "1", "path": ["9", "12", "976"], "permalink_uri": "https://repository.lib.tottori-u.ac.jp/records/4606", "pubdate": {"attribute_name": "公開日", "attribute_value": "2023-02-28"}, "publish_date": "2023-02-28", "publish_status": "0", "recid": "4606", "relation": {}, "relation_version_is_last": true, "title": ["Pathological Comparison of TDP-43 Between Motor Neurons and Interneurons Expressed by a Tetracycline Repressor System on the Mouse Artificial Chromosome"], "weko_shared_id": -1}