WEKO3
アイテム
Pathological Comparison of TDP-43 Between Motor Neurons and Interneurons Expressed by a Tetracycline Repressor System on the Mouse Artificial Chromosome
https://repository.lib.tottori-u.ac.jp/records/4606
https://repository.lib.tottori-u.ac.jp/records/460676ed80b1-3455-40c8-b304-f38042bc104d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
yam66(1)_24.pdf (6.2 MB)
|
|
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2023-02-28 | |||||
| タイトル | ||||||
| タイトル | Pathological Comparison of TDP-43 Between Motor Neurons and Interneurons Expressed by a Tetracycline Repressor System on the Mouse Artificial Chromosome | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | doxycycline | |||||
| キーワード | ||||||
| 主題 | interneuron | |||||
| キーワード | ||||||
| 主題 | motor neuron | |||||
| キーワード | ||||||
| 主題 | mouse artificial chromosome | |||||
| キーワード | ||||||
| 主題 | TAR-DNA binding protein of 43 kDa | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | doxycycline | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | interneuron | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | motor neuron | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | mouse artificial chromosome | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | TAR-DNA binding protein of 43 kDa | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
香月, 康宏
× 香月, 康宏× 平塚, 正治× Togai, Shota× Hamamichi, Shusei |
|||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Chromosome Biomedical Engineering, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Chromosome Engineering Research Center, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Chromosome Biomedical Engineering, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Sciences, Tottori University / Chromosome Engineering Research Center, Tottori University / Department of Chromosome Biomedical Engineering, School of Life Sciences, Faculty of Medicine, Tottori University / Chromosome Engineering Research Group, The Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Chromosome Engineering Research Center, Tottori University / Department of Chromosome Biomedical Engineering, School of Life Sciences, Faculty of Medicine, Tottori University | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Background: Cytoplasmic mislocalization of TAR-DNA binding protein of 43 kDa (TDP-43) is a major hallmark of amyotrophic lateral sclerosis (ALS). TDP-43 aggregation is detected in the cortical and spinal motor neurons in most ALS cases; however, pathological mechanism of this mislocalized TDP-43 remains unknown. Methods: We generated a tetracycline-inducible TDP-43 A315T system on a mouse artificial chromosome (MAC) vector to avoid transgene-insertional mutagenesis, established a mouse embryonic stem (ES) cell line holding this MAC vector system, and investigated whether overexpressed exogenous TDP-43 A315T was mislocalized in the cytoplasm of the ES cell-derived neurons and triggered the neurotoxic effects on these cells. Results: Inducible TDP-43 A315T system was successfully loaded onto the MAC and introduced into the mouse ES cells. These ES cells could differentiate into motor neurons and interneurons. Overexpression of TDP-43 A315T by addition of doxycycline in both neurons resulted in mislocalization to cytoplasm. Mislocalized TDP-43 caused cell death of motor neurons, but not interneurons. Conclusion: Vulnerability to cytoplasmic mislocalized TDP-43 is selective on neuronal types, whereas mislocalization of overexpressed TDP-43 occurs in even insusceptible neurons. This inducible gene expression system using MAC remains useful for providing critical insights into appearance of TDP-43 pathology. | |||||
| 書誌情報 |
Yonago Acta Medica en : Yonago Acta Medica 巻 66, 号 1, p. 24-35, 発行日 2023-02-20 |
|||||
| 出版者 | ||||||
| 出版者 | Tottori University Medical Press | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 05135710 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00892882 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.33160/yam.2023.02.004 | |||||
| 権利 | ||||||
| 権利情報 | (C) 2023 Tottori University Medical Press. | |||||
| 情報源 | ||||||
| 関連名称 | Yonago Acta Medica. 2023, 66(1), 24-35. doi:10.33160/yam.2023.02.004 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.lib.tottori-u.ac.jp/yam/yam/yam66-1/66-1contents.html | |||||
| 関連名称 | https://www.lib.tottori-u.ac.jp/yam/yam/yam66-1/66-1contents.html | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.33160/yam.2023.02.004 | |||||
| 関連名称 | https://doi.org/10.33160/yam.2023.02.004 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| EISSN | ||||||
| 値 | 13468049 | |||||
