{"_buckets": {"deposit": "090d0c65-2594-4275-8903-4b4499cb950b"}, "_deposit": {"created_by": 10, "id": "4629", "owners": [10], "pid": {"revision_id": 0, "type": "depid", "value": "4629"}, "status": "published"}, "_oai": {"id": "oai:repository.lib.tottori-u.ac.jp:00004629", "sets": ["12", "9", "979"]}, "author_link": ["3099", "4074", "16563"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2022-02-22", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "52", "bibliographicPageStart": "44", "bibliographicVolumeNumber": "65", "bibliographic_titles": [{"bibliographic_title": "Yonago Acta Medica"}, {"bibliographic_title": "Yonago Acta Medica", "bibliographic_titleLang": "en"}]}]}, "item_10001_checkbox_27": {"attribute_name": "部局名", "attribute_value_mlt": [{"subitem_checkbox_item": ["i-gakubu"]}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Background: Mammary serine protease inhibitor (maspin) is well known as a tumor suppressor gene in several types of cancers and its nuclear localization is essential for its tumor-suppressive function. We previously reported that the cytoplasmic-only localization of maspin is significantly correlated with unfavorable prognosis in patients with lung adenocarcinoma (LUAD). To clarify whether maspin in LUAD acts as a tumor promoter or suppressor, we examined the subcellular localization-dependent biological functions of maspin in human LUAD cell lines. Methods: The expression levels and subcellular localization of maspin were investigated by performing immunoblotting and immunofluorescence in human LUAD cell lines (PC-9, A549, NCI-H23, RERF-LCKJ) and human bronchial epithelial cell line (BEAS-2B). We then established stable cell lines overexpressing maspin (A549-maspin and RERF-LC-KJ-maspin) and investigated their subcellular localization. Cell invasion assays of these cell lines were performed to examine their invasiveness. Moreover, the mRNA expression levels between epithelial cell markers (E-cadherin) and mesenchymal cell markers (N-cadherin and vimentin) were compared. Results: The expression of maspin in PC-9 cells was comparable to that in BEAS-2B cells, whereas its expression in A549, NCI-H23, and RERF-LC-KJ cells was decreased. The cell invasion capability of A549-maspin cells showing pancellular expression was significantly decreased compared with that of A549-control cells. By contrast, the cell invasion capability of RERF-LC-KJmaspin cells showing cytoplasmic-only expression was significantly increased compared with that of RERFLC-KJ-control cells. The mRNA expression levels of N-cadherin, but not E-cadherin and vimentin, in A549-maspin cells was significantly downregulated compared with that in A549-control cells. No significant differences in these markers were observed between RERFLC-KJ-maspin and RERF-LC-KJ-control cells. Conclusion: The invasive capability of LUAD cells is regulated by the intracellular localization of maspin. Clarification of the molecular mechanism underlying the subcellular localization-dependent function of maspin will promote a deeper understanding of LUAD development and progression.", "subitem_description_type": "Other"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Tottori University Medical Press"}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.33160/yam.2022.02.006", "subitem_relation_type_select": "DOI"}}]}, "item_10001_relation_16": {"attribute_name": "情報源", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "Yonago Acta Medica. 2022, 65(1), 44-52. doi:10.33160/yam.2022.02.006"}]}]}, "item_10001_relation_17": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": 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{"attribute_name": "EARMAS ID", "attribute_value_mlt": [{"subitem_text_value": "13696"}]}, "item_10001_text_26": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_text_value": "13468049"}]}, "item_10001_text_29": {"attribute_name": "最新掲載誌名", "attribute_value_mlt": [{"subitem_text_value": "yam;Yonago Acta medica;Yonago Acta medica"}]}, "item_10001_text_33": {"attribute_name": "著者所属（英）", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Department of Pathology, School of Medicine, Faculty of Medicine, Tottori University"}, {"subitem_text_language": "en", "subitem_text_value": "Department of Pathology, School of Medicine, Faculty of Medicine, Tottori University"}, {"subitem_text_language": "en", "subitem_text_value": "Department of Pathology, School of Medicine, Faculty of Medicine, Tottori University"}]}, "item_10001_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "坂部, 友彦"}, {"creatorName": "サカベ, トモヒコ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Sakabe, Tomohiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "3099", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "50639747", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=50639747"}, {"nameIdentifier": "100001095", "nameIdentifierScheme": "研究者総覧鳥取大学", "nameIdentifierURI": "http://researchers.adm.tottori-u.ac.jp/html/100001095_ja.html"}]}, {"creatorNames": [{"creatorName": "梅北, 善久"}, {"creatorName": "ウメキタ, ヨシヒサ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Umekita, Yoshihisa", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4074", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "80244226", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=80244226"}, {"nameIdentifier": "100000852", "nameIdentifierScheme": "研究者総覧鳥取大学", "nameIdentifierURI": "http://researchers.adm.tottori-u.ac.jp/html/100000852_ja.html"}]}, {"creatorNames": [{"creatorName": "Matsushige, Takahiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "16563", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2023-03-15"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "yam65(1)_44.pdf", "filesize": [{"value": "3.6 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "(C) 2022 Tottori University Medical Press.", "licensetype": "license_note", "mimetype": "application/pdf", "size": 3600000.0, "url": {"label": "yam65(1)_44.pdf", "url": "https://repository.lib.tottori-u.ac.jp/record/4629/files/yam65(1)_44.pdf"}, "version_id": "f532337c-a6ff-46df-9235-4ddeb525c992"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "lung adenocarcinoma", "subitem_subject_scheme": "Other"}, {"subitem_subject": "maspin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "lung adenocarcinoma", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "maspin", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Investigation of the Subcellular Localization-Dependent Anti- or Pro-Tumor Functions of Maspin in Human Lung Adenocarcinoma Cell Line", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Investigation of the Subcellular Localization-Dependent Anti- or Pro-Tumor Functions of Maspin in Human Lung Adenocarcinoma Cell Line", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "10", "path": ["12", "9", "979"], "permalink_uri": "https://repository.lib.tottori-u.ac.jp/records/4629", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2022-03-09"}, "publish_date": "2022-03-09", "publish_status": "0", "recid": "4629", "relation": {}, "relation_version_is_last": true, "title": ["Investigation of the Subcellular Localization-Dependent Anti- or Pro-Tumor Functions of Maspin in Human Lung Adenocarcinoma Cell Line"], "weko_shared_id": -1}