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  1. 学部学科区分一覧
  2. 医学部・医学系研究科・医学部附属病院
  1. 資料タイプ一覧
  2. 学術雑誌論文
  1. 鳥取大学の刊行物
  2. Yonago Acta Medica
  3. 65
  4. 1

Investigation of the Subcellular Localization-Dependent Anti- or Pro-Tumor Functions of Maspin in Human Lung Adenocarcinoma Cell Line

https://repository.lib.tottori-u.ac.jp/records/4629
https://repository.lib.tottori-u.ac.jp/records/4629
bdba72e2-360c-400c-9511-37bc2cbd40b0
名前 / ファイル ライセンス アクション
yam65(1)_44.pdf yam65(1)_44.pdf (3.6 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2022-03-09
タイトル
タイトル Investigation of the Subcellular Localization-Dependent Anti- or Pro-Tumor Functions of Maspin in Human Lung Adenocarcinoma Cell Line
言語 en
言語
言語 eng
キーワード
主題 lung adenocarcinoma
キーワード
主題 maspin
キーワード
言語 en
主題 lung adenocarcinoma
キーワード
言語 en
主題 maspin
資源タイプ
資源タイプ journal article
著者 坂部, 友彦

× 坂部, 友彦

WEKO 3099
e-Rad 50639747
研究者総覧鳥取大学 100001095

坂部, 友彦

ja-Kana サカベ, トモヒコ

en Sakabe, Tomohiko

Search repository
梅北, 善久

× 梅北, 善久

WEKO 4074
e-Rad 80244226
研究者総覧鳥取大学 100000852

梅北, 善久

ja-Kana ウメキタ, ヨシヒサ

en Umekita, Yoshihisa

Search repository
Matsushige, Takahiro

× Matsushige, Takahiro

WEKO 16563

en Matsushige, Takahiro

Search repository
著者所属(英)
言語 en
値 Department of Pathology, School of Medicine, Faculty of Medicine, Tottori University
著者所属(英)
言語 en
値 Department of Pathology, School of Medicine, Faculty of Medicine, Tottori University
著者所属(英)
言語 en
値 Department of Pathology, School of Medicine, Faculty of Medicine, Tottori University
抄録
内容記述タイプ Other
内容記述 Background: Mammary serine protease inhibitor (maspin) is well known as a tumor suppressor gene in several types of cancers and its nuclear localization is essential for its tumor-suppressive function. We previously reported that the cytoplasmic-only localization of maspin is significantly correlated with unfavorable prognosis in patients with lung adenocarcinoma (LUAD). To clarify whether maspin in LUAD acts as a tumor promoter or suppressor, we examined the subcellular localization-dependent biological functions of maspin in human LUAD cell lines. Methods: The expression levels and subcellular localization of maspin were investigated by performing immunoblotting and immunofluorescence in human LUAD cell lines (PC-9, A549, NCI-H23, RERF-LCKJ) and human bronchial epithelial cell line (BEAS-2B). We then established stable cell lines overexpressing maspin (A549-maspin and RERF-LC-KJ-maspin) and investigated their subcellular localization. Cell invasion assays of these cell lines were performed to examine their invasiveness. Moreover, the mRNA expression levels between epithelial cell markers (E-cadherin) and mesenchymal cell markers (N-cadherin and vimentin) were compared. Results: The expression of maspin in PC-9 cells was comparable to that in BEAS-2B cells, whereas its expression in A549, NCI-H23, and RERF-LC-KJ cells was decreased. The cell invasion capability of A549-maspin cells showing pancellular expression was significantly decreased compared with that of A549-control cells. By contrast, the cell invasion capability of RERF-LC-KJmaspin cells showing cytoplasmic-only expression was significantly increased compared with that of RERFLC-KJ-control cells. The mRNA expression levels of N-cadherin, but not E-cadherin and vimentin, in A549-maspin cells was significantly downregulated compared with that in A549-control cells. No significant differences in these markers were observed between RERFLC-KJ-maspin and RERF-LC-KJ-control cells. Conclusion: The invasive capability of LUAD cells is regulated by the intracellular localization of maspin. Clarification of the molecular mechanism underlying the subcellular localization-dependent function of maspin will promote a deeper understanding of LUAD development and progression.
書誌情報 Yonago Acta Medica
en : Yonago Acta Medica

巻 65, 号 1, p. 44-52, 発行日 2022-02-22
出版者
出版者 Tottori University Medical Press
ISSN
収録物識別子タイプ ISSN
収録物識別子 05135710
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00892882
DOI
関連タイプ isIdenticalTo
識別子タイプ DOI
関連識別子 10.33160/yam.2022.02.006
権利
権利情報 (C) 2022 Tottori University Medical Press.
情報源
関連名称 Yonago Acta Medica. 2022, 65(1), 44-52. doi:10.33160/yam.2022.02.006
関連サイト
識別子タイプ URI
関連識別子 https://www.lib.tottori-u.ac.jp/yam/yam/yam65-1/65-1contents.html
関連名称 https://www.lib.tottori-u.ac.jp/yam/yam/yam65-1/65-1contents.html
関連サイト
識別子タイプ DOI
関連識別子 https://doi.org/10.33160/yam.2022.02.006
関連名称 https://doi.org/10.33160/yam.2022.02.006
著者版フラグ
出版タイプ VoR
EISSN
値 13468049
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