WEKO3
アイテム
Cell Death Induction of Thymidine Kinase Gene Transfer Followed by Ganciclovir Treatment in Oral Squamous Cell Carcinoma Cell Lines
https://repository.lib.tottori-u.ac.jp/records/5082
https://repository.lib.tottori-u.ac.jp/records/50821011d189-d230-487d-97c6-6bb2f76b24fe
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
48_063-074.pdf (326.7 kB)
|
|
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-06-22 | |||||
| タイトル | ||||||
| タイトル | Cell Death Induction of Thymidine Kinase Gene Transfer Followed by Ganciclovir Treatment in Oral Squamous Cell Carcinoma Cell Lines | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | apoptosis | |||||
| キーワード | ||||||
| 主題 | ganciclovir | |||||
| キーワード | ||||||
| 主題 | necrosis | |||||
| キーワード | ||||||
| 主題 | thymidine kinase | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | apoptosis | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | ganciclovir | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | necrosis | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | thymidine kinase | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Kuratate, Itaru
× Kuratate, Itaru× Osaki, Mitsuhiko× Ryoke, Kazuo× Kuratate, Itaru |
|||||
| 著者所属 | ||||||
| 値 | Division of Organ Pathology, Department of Microbiology and Pathology,Tottori University Faculty of Medicine. Division of Oral and Maxillofacial Biopathological Surgery, Department of Medicine of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine | |||||
| 著者所属 | ||||||
| 値 | Division of Organ Pathology, Department of Microbiology and Pathology,Tottori University Faculty of Medicine | |||||
| 著者所属 | ||||||
| 値 | Division of Oral and Maxillofacial Biopathological Surgery, Department of Medicine of Sensory and Motor Organs, School of Medicine, Tottori University Faculty of Medicine | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The tumoricidal effect of herpes simplex virus thymidine kinase (HSV-tk) gene transfer followed by ganciclovir (GCV) treatment has been demonstrated in relation to the bystander effect. We examined the mode of cell death after GCV treatment in three human oral squamous cell carcinoma (SCC) cell lines that had been infected with adenovirus possessing HSV-tk gene. Oral SCC cell lines displayed high susceptibility to HSV-tk/GCV treatment despite low transduction. Evidence suggests that apoptosis was not found by internucleosomal DNA fragmentation, cell cycle kinetics or gene expressions indicative of apoptosis. However, we observed different levels of expression of annexin V-positive/propidium iodide-negative cells in all the cell lines and occasional TUNEL-positive cells in one cell line. To address these controversial findings, we further confirmed the morphological phenotypes of cell death with semi-thin and ultra-thin sections, which revealed that the cells undergoing death were consistent with necrosis, i.e. swelling of cytoplasm and intracellular organs with membrane disruption. Furthermore, no apoptotic bodies were detected within cytoplasm of apparently intact cells. A nonapoptotic mechanism may play a central role in the HSV-tk/GCV-induced cell death in oral SCC cell lines. | |||||
| 書誌情報 |
Yonago Acta medica en : Yonago Acta medica 巻 48, 号 3, p. 63-74, 発行日 2005-09 |
|||||
| 出版者 | ||||||
| 出版者 | Tottori University Faculty of Medicine | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13468049 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00892882 | |||||
| 権利 | ||||||
| 権利情報 | Yonago Acta medica 編集委員会 | |||||
| 情報源 | ||||||
| 関連名称 | Yonago Acta medica. 2005, 48(3), 63-74 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
