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An Inhibitory Effect of Dexamethasone on A549 Cell Adhesion to Neutrophils: Possible Involvement of Nuclear Factor-Kappa B
https://repository.lib.tottori-u.ac.jp/records/5167
https://repository.lib.tottori-u.ac.jp/records/51676fa5256e-2d81-46a9-a891-73a4d214e379
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
42_011-020.pdf (220.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2018-06-22 | |||||
| タイトル | ||||||
| タイトル | An Inhibitory Effect of Dexamethasone on A549 Cell Adhesion to Neutrophils: Possible Involvement of Nuclear Factor-Kappa B | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | alveolar epithelial cells | |||||
| キーワード | ||||||
| 主題 | adhesion assay | |||||
| キーワード | ||||||
| 主題 | ICAM-1 | |||||
| キーワード | ||||||
| 主題 | laser scanning cytometry | |||||
| キーワード | ||||||
| 主題 | neutrophils | |||||
| キーワード | ||||||
| 主題 | NF-κB | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | alveolar epithelial cells | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | adhesion assay | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | ICAM-1 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | laser scanning cytometry | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | neutrophils | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | NF-κB | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Tokuyasu, Hirokazu
× Tokuyasu, Hirokazu× Tomita, Katsuyuki× Hitsuda, Yutaka× Tokuyasu, Hirokazu× Tomita, Katsuyuki× Hitsuda, Yutaka |
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| 著者所属 | ||||||
| 値 | Third Department of Internal Medicine, Faculty of Medicine, Tottori University | |||||
| 著者所属 | ||||||
| 値 | Third Department of Internal Medicine, Faculty of Medicine, Tottori University | |||||
| 著者所属 | ||||||
| 値 | Third Department of Internal Medicine, Faculty of Medicine, Tottori University | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | In order to clarify the effects of dexamethasone on epithelial cells at the transcription factor level, protein synthesis level and functional level, we examined neutrophil adhesion to A549 cells (an immortalized human type II alveolar epithelial cell line), expression of intercellular adhesion molecule-1 (ICAM-1), and translocation of nuclear factor-kappa B (NF-κB) into nuclei. The intranuclear localization of fluorescently stained NF-κB and an adhesion assay between neutrophils and A549 cells was analyzed quantitatively using laser scanning cytometry. ICAM-1 expression on A549 cells was measured by flow cytometric analysis. Stimulation of A549 cells with tumor necrosis factor (TNF)-α caused a time- and dose-dependent increase in their adhesiveness for neutrophils. ICAM-1 expression on A549 cells after stimulation with TNF-α for 12 h was significantly up-regulated, compared with unstimulated cells (P< 0.05). Intranuclear NF-κB was induced 10 min after stimulation with TNF-α and was increased in a TNF-α-dose-dependent manner. Dexamethasone suppressed TNF-α-induced NF-κB translocation in A549 cells by approximately 60%, ICAM-1 expression on A549 cells by approximately 40%, and A549 adhesiveness for neutrophils completely. Therefore, we suggest that the inhibition of A549 adhesiveness for neutrophils by dexamethasone may be due in part to the suppression of NF-κB entry into nuclei and ICAM-1 expression in A549 cells. | |||||
| 書誌情報 |
Yonago Acta medica en : Yonago Acta medica 巻 42, 号 1, p. 11-20, 発行日 1999-03 |
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| 出版者 | ||||||
| 出版者 | Tottori University Faculty of Medicine | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13468049 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00892882 | |||||
| 権利 | ||||||
| 権利情報 | Yonago Acta medica 編集委員会 | |||||
| 情報源 | ||||||
| 関連名称 | Yonago Acta medica. 1999, 42(1), 11-20 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
