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Activated Protein Kinase C Attenuates Ca2+ Overloading and Reoxygenation Hypercontracture in Isolated Rat Cardiomyocytes following Chemical Hypoxia
https://repository.lib.tottori-u.ac.jp/records/5169
https://repository.lib.tottori-u.ac.jp/records/5169d36714ef-90be-4a9c-a7d3-59361c48833b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
42_031-039.pdf (82.8 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2018-06-22 | |||||
| タイトル | ||||||
| タイトル | Activated Protein Kinase C Attenuates Ca2+ Overloading and Reoxygenation Hypercontracture in Isolated Rat Cardiomyocytes following Chemical Hypoxia | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | Ca2+ overload | |||||
| キーワード | ||||||
| 主題 | intracellular pH | |||||
| キーワード | ||||||
| 主題 | isolated rat cardiomyocytes | |||||
| キーワード | ||||||
| 主題 | Na+-Ca2+ exchanger | |||||
| キーワード | ||||||
| 主題 | protein kinase C | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | Ca2+ overload | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | intracellular pH | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | isolated rat cardiomyocytes | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | Na+-Ca2+ exchanger | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | protein kinase C | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Miyasaka, Shigeto
× Miyasaka, Shigeto× Ichiba, Takashi× Kuroda, Hiroaki× Ohgi, Shigetsugu× Miyasaka, Shigeto× Ichiba, Takashi× Kuroda, Hiroaki |
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| 著者所属 | ||||||
| 値 | Second Department of Surgery, Faculty of Medicine, Tottori University | |||||
| 著者所属 | ||||||
| 値 | Second Department of Surgery, Faculty of Medicine, Tottori University | |||||
| 著者所属 | ||||||
| 値 | Second Department of Surgery, Faculty of Medicine, Tottori University | |||||
| 著者所属 | ||||||
| 値 | Second Department of Surgery, Faculty of Medicine, Tottori University | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The aims of this study were i) to test the effects of protein kinase C (PKC) activation on the intracellular Ca2+ concentration of rat cardiomyocytes during chemical hypoxia-reoxygenation, ii) to determine the contribution of the sarcoplasmic reticulum (SR) and the Na+-Ca2+ exchange on the regulation of intracellular Ca2+ following PKC activation and iii) to test the role of PKC-dependent intracellular pH changes in intracellular Ca2+ regulation. We used the isolated adult rat cardiomyocyte perfusion model. Cardiomyocytes were loaded with the Ca2+-fluorescent probe Fluo-3 and the pH-fluorescent probe SNARF1. Cells were subjected to 50 min of glucose-free and NaCN chemical hypoxia followed by 30 min of simulated reoxygenation. The activation of PKC significantly inhibited the hypoxia-induced increase of Fluo-3 fluorescent intensity (control; 692 ± 100% and activated PKC; 322 ± 43%: P < 0.05). This inhibitory effect was not affected by the inhibition of the SR Ca2+ uptake induced by thapsigargin, but was cancelled by the inhibition of the Na+-Ca2+ exchange with dichlorobenzamil (thapsigargin 337 ± 47%; dichlorobenzamil 609 ± 100%). PKC activation also attenuated the decrease in intracellular pH during chemical hypoxia, even in the presence of the Na+-H+ exchange inhibitor amiloride (control; 6.54 ± 0.02, PKC; 6.72 ± 0.03, PKC + amiloride; 6.73 ± 0.03). We concluded that the PKC attenuation of Ca2+ overloading to rat cardiomyocytes during chemical hypoxia-reoxygenation does not depend on the Ca2+ uptake by the SR, but does require a Na+-Ca2+ exchange. Since PKC attenuated the increasing intracellular H+ during chemical hypoxia, a low H+ concentration may be important for the maintenance of Ca2+ extrusion via the Na+-Ca2+ exchange. | |||||
| 書誌情報 |
Yonago Acta medica en : Yonago Acta medica 巻 42, 号 1, p. 31-39, 発行日 1999-03 |
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| 出版者 | ||||||
| 出版者 | Tottori University Faculty of Medicine | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13468049 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00892882 | |||||
| 権利 | ||||||
| 権利情報 | Yonago Acta medica 編集委員会 | |||||
| 情報源 | ||||||
| 関連名称 | Yonago Acta medica. 1999, 42(1), 31-39 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
