Item type |
学術雑誌論文 / Journal Article(1) |
公開日 |
2018-06-22 |
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タイトル |
Effects of Extracellular Acidification on Intracellular pH and ATP-Induced Calcium Mobilization in Rabbit Lens Epithelial Cells |
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言語 |
en |
言語 |
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言語 |
eng |
キーワード |
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主題Scheme |
Other |
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主題 |
ATP-induced calcium mobilization |
キーワード |
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主題Scheme |
Other |
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主題 |
extracellular acidification |
キーワード |
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主題Scheme |
Other |
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主題 |
intracellular pH |
キーワード |
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主題Scheme |
Other |
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主題 |
rabbit lens epithelial cells |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
ATP-induced calcium mobilization |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
extracellular acidification |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
intracellular pH |
キーワード |
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言語 |
en |
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主題Scheme |
Other |
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主題 |
rabbit lens epithelial cells |
資源タイプ |
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資源タイプ |
journal article |
著者 |
Narita, Akiko
Yawata, Kenji
Nagata, Masao
Aoyama, Yasuaki
Kawai, Yasuaki
Narita, Akiko
Yawata, Kenji
Nagata, Masao
Aoyama, Yasuaki
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著者所属 |
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値 |
Second Department of Physiology and Department of Ophthalmology, Faculty of Medicine, Tottori University |
著者所属 |
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値 |
Second Department of Physiology and Department of Ophthalmology, Faculty of Medicine, Tottori University |
著者所属 |
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値 |
Department of Ophthalmology, Faculty of Medicine, Tottori University |
著者所属 |
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値 |
Second Department of Physiology, Faculty of Medicine, Tottori University |
著者所属 |
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値 |
Second Department of Physiology, Faculty of Medicine, Tottori University |
抄録 |
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内容記述タイプ |
Other |
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内容記述 |
Effects of extracellular acidification on intracellular pH (pHi) and ATP-induced calcium mobilization were investigated in rabbit lens epithelial cells. Primary-cultured lens epithelial cells of Japanese white rabbits were used. Intracellular calcium ([Ca2+]i) and pHi were measured by using fluorescent dyes, fura-2 acetoxymethylester (fura-2 AM) and 2',7'-bis (carboxyethyl)-5,6-carboxyfluorescein acetoxymethylester (BCECF AM), respectively. The addition of 10 ?mol/L ATP produced an initial peak followed by a sustained increase in [Ca2+]i in a standard artificial aqueous humor at extracellular pH (pH0) 7.40. The initial peak was abolished by pretreatment with 1 ?mol/L thapsigargin, whereas the sustained increase was attenuated in a Ca2+-free solution or by pretreatment with 100 ?mol/L verapamil. Acidification of the pH0 from 7.40 to 6.80 decreased the pHi from 7.21 to 7.03, and enhanced both the initial peak and sustained increase in [Ca2+]i. These results suggest that acidification of pH0 significantly affects the pHi and modifies the ATP-induced [Ca2+]i transient in rabbit lens epithelial cells. |
書誌情報 |
Yonago Acta medica
en : Yonago Acta medica
巻 42,
号 1,
p. 51-59,
発行日 1999-03
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出版者 |
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出版者 |
Tottori University Faculty of Medicine |
ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
13468049 |
書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA00892882 |
権利 |
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権利情報 |
Yonago Acta medica 編集委員会 |
情報源 |
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関連名称 |
Yonago Acta medica. 1999, 42(1), 51-59 |
著者版フラグ |
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出版タイプ |
VoR |