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Ethanol Induces Apoptosis in Human Gastric Carcinoma Cells: The Role of Apoptosis-Related Molecules
https://repository.lib.tottori-u.ac.jp/records/5178
https://repository.lib.tottori-u.ac.jp/records/5178dc7fbef6-a59c-4493-ae3e-e5dfddfd897f
| 名前 / ファイル | ライセンス | アクション |
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42_113-124.pdf (109.5 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2018-06-22 | |||||
| タイトル | ||||||
| タイトル | Ethanol Induces Apoptosis in Human Gastric Carcinoma Cells: The Role of Apoptosis-Related Molecules | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | apoptosis | |||||
| キーワード | ||||||
| 主題 | gastric carcinoma cell line | |||||
| キーワード | ||||||
| 主題 | ethanol | |||||
| キーワード | ||||||
| 主題 | calcium | |||||
| キーワード | ||||||
| 主題 | c-Myc protein | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | apoptosis | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | gastric carcinoma cell line | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | ethanol | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | calcium | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | c-Myc protein | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
Kasagi, Noriko
× Kasagi, Noriko |
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| 著者所属 | ||||||
| 値 | First Department of Pathology, Faculty of Medicine, Tottori University | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Ethanol triggers apoptosis in a variety of mammalian cultured cells. Here, ethanol-induced apoptosis in five human gastric carcinoma cell lines was examined. Trypan blue-positive cells were detected in more than 77% of 10% ethanol treated cells at 6 h in all the cell lines. The apoptosis, which had a typical morphology and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) signals were induced with 3% and 5% ethanol treatments. The apoptotic cells were detected in 36% of MKN-74, 54% of MKN-45 which have wild-type p53, and 37% of TMK-1 cells at 48 h with 5% ethanol, in contrast to less than 18% of MKN-28 and KATO-III cells. The DNA ladder appeared in the MKN-74, MKN-45 and TMK-1, but not in the MKN-28 and KATO-III. The expression of apoptosis-associated molecules p53, Bcl-2, Bax and c-Myc proteins was analyzed in the ethanol-treated cells. The levels of p53, Bcl-2 and Bax proteins were not changed in the ethanol-induced apoptosis. MKN-28 and KATO-III showed higher expressions of Bcl-2 protein compared to the other cell lines, and the down-regulation of c-Myc protein after 1.5 h of ethanol treatment. Calcium antagonists verapamil or nifedipine decreased apoptotic cells in ethanol treated MKN-74, MKN-45 and TMK-1. These results suggest: i) 3% and 5% ethanol induced apoptosis via the influx of extracellular calcium, but were not changed the expression levels of p53 and c-Myc protein, while 10% ethanol provoked necrosis in these cell lines, and ii) the apoptosis resistance by ethanol might be implicated in the higher expression of Bcl-2 protein and down-regulation of c-Myc protein as shown in MKN-28 and KATO-III. | |||||
| 書誌情報 |
Yonago Acta medica en : Yonago Acta medica 巻 42, 号 1, p. 113-124, 発行日 1999-03 |
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| 出版者 | ||||||
| 出版者 | Tottori University Faculty of Medicine | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13468049 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00892882 | |||||
| 権利 | ||||||
| 権利情報 | Yonago Acta medica 編集委員会 | |||||
| 情報源 | ||||||
| 関連名称 | Yonago Acta medica. 1999, 42(1), 113-124 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
