{"_buckets": {"deposit": "33ea54d7-be56-4b3c-94c1-b5e641a17048"}, "_deposit": {"created_by": 10, "id": "5178", "owners": [10], "pid": {"revision_id": 0, "type": "depid", "value": "5178"}, "status": "published"}, "_oai": {"id": "oai:repository.lib.tottori-u.ac.jp:00005178", "sets": ["12", "9", "1092"]}, "author_link": ["1319"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1999-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "124", "bibliographicPageStart": "113", "bibliographicVolumeNumber": "42", "bibliographic_titles": [{"bibliographic_title": "Yonago Acta medica"}, {"bibliographic_title": "Yonago Acta medica", "bibliographic_titleLang": "en"}]}]}, "item_10001_checkbox_27": {"attribute_name": "部局名", "attribute_value_mlt": [{"subitem_checkbox_item": ["i-gakubu"]}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Ethanol triggers apoptosis in a variety of mammalian cultured cells. Here, ethanol-induced apoptosis in five human gastric carcinoma cell lines was examined. Trypan blue-positive cells were detected in more than 77% of 10% ethanol treated cells at 6 h in all the cell lines. The apoptosis, which had a typical morphology and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) signals were induced with 3% and 5% ethanol treatments. The apoptotic cells were detected in 36% of MKN-74, 54% of MKN-45 which have wild-type p53, and 37% of TMK-1 cells at 48 h with 5% ethanol, in contrast to less than 18% of MKN-28 and KATO-III cells. The DNA ladder appeared in the MKN-74, MKN-45 and TMK-1, but not in the MKN-28 and KATO-III. The expression of apoptosis-associated molecules p53, Bcl-2, Bax and c-Myc proteins was analyzed in the ethanol-treated cells. The levels of p53, Bcl-2 and Bax proteins were not changed in the ethanol-induced apoptosis. MKN-28 and KATO-III showed higher expressions of Bcl-2 protein compared to the other cell lines, and the down-regulation of c-Myc protein after 1.5 h of ethanol treatment. Calcium antagonists verapamil or nifedipine decreased apoptotic cells in ethanol treated MKN-74, MKN-45 and TMK-1. These results suggest: i) 3% and 5% ethanol induced apoptosis via the influx of extracellular calcium, but were not changed the expression levels of p53 and c-Myc protein, while 10% ethanol provoked necrosis in these cell lines, and ii) the apoptosis resistance by ethanol might be implicated in the higher expression of Bcl-2 protein and down-regulation of c-Myc protein as shown in MKN-28 and KATO-III.", "subitem_description_type": "Other"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Tottori University Faculty of Medicine"}]}, "item_10001_relation_16": {"attribute_name": "情報源", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "Yonago Acta medica. 1999, 42(1), 113-124"}]}]}, "item_10001_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Yonago Acta medica　編集委員会"}]}, "item_10001_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00892882", "subitem_source_identifier_type": "NCID"}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "13468049", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_text_25": {"attribute_name": "EARMAS ID", "attribute_value_mlt": [{"subitem_text_value": "918"}]}, "item_10001_text_29": {"attribute_name": "最新掲載誌名", "attribute_value_mlt": [{"subitem_text_value": "yam;Yonago Acta medica;Yonago Acta medica"}]}, "item_10001_text_32": {"attribute_name": "著者所属", "attribute_value_mlt": [{"subitem_text_value": "First Department of Pathology, Faculty of Medicine, Tottori University"}]}, "item_10001_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kasagi, Noriko"}, {"creatorName": "Kasagi, Noriko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1319", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "60185741", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=60185741"}, {"nameIdentifier": "100000123", "nameIdentifierScheme": "研究者総覧鳥取大学", "nameIdentifierURI": "http://researchers.adm.tottori-u.ac.jp/html/100000123_ja.html"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2009-06-08"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "42_113-124.pdf", "filesize": [{"value": "109.5 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "Yonago Acta medica　編集委員会", "licensetype": "license_note", "mimetype": "application/pdf", "size": 109500.0, "url": {"label": "42_113-124.pdf", "url": "https://repository.lib.tottori-u.ac.jp/record/5178/files/42_113-124.pdf"}, "version_id": "350d7349-5d70-4bcd-b00b-e353fdaee6e5"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "apoptosis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "gastric carcinoma cell line", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ethanol", "subitem_subject_scheme": "Other"}, {"subitem_subject": "calcium", "subitem_subject_scheme": "Other"}, {"subitem_subject": "c-Myc protein", "subitem_subject_scheme": "Other"}, {"subitem_subject": "apoptosis", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "gastric carcinoma cell line", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ethanol", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "calcium", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "c-Myc protein", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Ethanol Induces Apoptosis in Human Gastric Carcinoma Cells: The Role of Apoptosis-Related Molecules", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Ethanol Induces Apoptosis in Human Gastric Carcinoma Cells: The Role of Apoptosis-Related Molecules", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "10", "path": ["12", "9", "1092"], "permalink_uri": "https://repository.lib.tottori-u.ac.jp/records/5178", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2018-06-22"}, "publish_date": "2018-06-22", "publish_status": "0", "recid": "5178", "relation": {}, "relation_version_is_last": true, "title": ["Ethanol Induces Apoptosis in Human Gastric Carcinoma Cells: The Role of Apoptosis-Related Molecules"], "weko_shared_id": -1}