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Ethanol Induces Apoptosis in Human Gastric Carcinoma Cells: The Role of Apoptosis-Related Molecules
https://repository.lib.tottori-u.ac.jp/records/5178
https://repository.lib.tottori-u.ac.jp/records/5178dc7fbef6-a59c-4493-ae3e-e5dfddfd897f
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-06-22 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Ethanol Induces Apoptosis in Human Gastric Carcinoma Cells: The Role of Apoptosis-Related Molecules | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | apoptosis | |||||
キーワード | ||||||
主題 | gastric carcinoma cell line | |||||
キーワード | ||||||
主題 | ethanol | |||||
キーワード | ||||||
主題 | calcium | |||||
キーワード | ||||||
主題 | c-Myc protein | |||||
キーワード | ||||||
言語 | en | |||||
主題 | apoptosis | |||||
キーワード | ||||||
言語 | en | |||||
主題 | gastric carcinoma cell line | |||||
キーワード | ||||||
言語 | en | |||||
主題 | ethanol | |||||
キーワード | ||||||
言語 | en | |||||
主題 | calcium | |||||
キーワード | ||||||
言語 | en | |||||
主題 | c-Myc protein | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
著者 |
Kasagi, Noriko
× Kasagi, Noriko |
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著者所属 | ||||||
First Department of Pathology, Faculty of Medicine, Tottori University | ||||||
抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Ethanol triggers apoptosis in a variety of mammalian cultured cells. Here, ethanol-induced apoptosis in five human gastric carcinoma cell lines was examined. Trypan blue-positive cells were detected in more than 77% of 10% ethanol treated cells at 6 h in all the cell lines. The apoptosis, which had a typical morphology and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) signals were induced with 3% and 5% ethanol treatments. The apoptotic cells were detected in 36% of MKN-74, 54% of MKN-45 which have wild-type p53, and 37% of TMK-1 cells at 48 h with 5% ethanol, in contrast to less than 18% of MKN-28 and KATO-III cells. The DNA ladder appeared in the MKN-74, MKN-45 and TMK-1, but not in the MKN-28 and KATO-III. The expression of apoptosis-associated molecules p53, Bcl-2, Bax and c-Myc proteins was analyzed in the ethanol-treated cells. The levels of p53, Bcl-2 and Bax proteins were not changed in the ethanol-induced apoptosis. MKN-28 and KATO-III showed higher expressions of Bcl-2 protein compared to the other cell lines, and the down-regulation of c-Myc protein after 1.5 h of ethanol treatment. Calcium antagonists verapamil or nifedipine decreased apoptotic cells in ethanol treated MKN-74, MKN-45 and TMK-1. These results suggest: i) 3% and 5% ethanol induced apoptosis via the influx of extracellular calcium, but were not changed the expression levels of p53 and c-Myc protein, while 10% ethanol provoked necrosis in these cell lines, and ii) the apoptosis resistance by ethanol might be implicated in the higher expression of Bcl-2 protein and down-regulation of c-Myc protein as shown in MKN-28 and KATO-III. | |||||
書誌情報 |
Yonago Acta medica en : Yonago Acta medica 巻 42, 号 1, p. 113-124, 発行日 1999-03 |
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出版者 | ||||||
出版者 | Tottori University Faculty of Medicine | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13468049 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00892882 | |||||
権利 | ||||||
権利情報 | Yonago Acta medica 編集委員会 | |||||
情報源 | ||||||
関連名称 | Yonago Acta medica. 1999, 42(1), 113-124 | |||||
著者版フラグ | ||||||
出版タイプ | VoR |