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Germ Tube Formation of Candida albicans in Corn Meal Broth Using the Non-Slip Slide Glass Incubation Method
https://repository.lib.tottori-u.ac.jp/records/5204
https://repository.lib.tottori-u.ac.jp/records/5204722f8041-8451-4474-8183-2b75fe0f930c
名前 / ファイル | ライセンス | アクション |
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41_065-072.pdf (90.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-06-22 | |||||
タイトル | ||||||
タイトル | Germ Tube Formation of Candida albicans in Corn Meal Broth Using the Non-Slip Slide Glass Incubation Method | |||||
言語 | en | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | Candida albicans | |||||
キーワード | ||||||
主題 | corn meal broth | |||||
キーワード | ||||||
主題 | germ tube formation | |||||
キーワード | ||||||
主題 | non-slip slide glass incubation method | |||||
キーワード | ||||||
言語 | en | |||||
主題 | Candida albicans | |||||
キーワード | ||||||
言語 | en | |||||
主題 | corn meal broth | |||||
キーワード | ||||||
言語 | en | |||||
主題 | germ tube formation | |||||
キーワード | ||||||
言語 | en | |||||
主題 | non-slip slide glass incubation method | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
著者 |
Nakamoto, Sachiko
× Nakamoto, Sachiko |
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著者所属 | ||||||
値 | Department of Medical Technology, Tottori University, Institute of Medical Care Technology | |||||
抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Candida albicans IFO 1385 yeast cells dominantly develop germ tubes in a dilute corn meal broth at 37°C. For germ tube (GT) development the broth concentration is optimal at 75% of the original, and the inoculum is optimal at 105 cells/mL. Incubation procedures were compared and the non-slip slide glass incubation (NSSI) method was found to be more available than the tube incubation method or slid-covered slide glass incubation method. The GT formation was completed in 1 to 2 h in the NSSI method and clear-cut GTs were observed under optical microscopy. GTs were formed in all 111 test-strains of C. albicans but not at all in other species of Candida. Therefore, under optimal conditions, the rate of identifying C. albicans is 100%, demonstrating that GT detection with the NSSI method is a rapid and reliable identification method. The humid NSSI method is suited for continuous observation of a GT. The initially formed GT elongated to resemble a true hypha, attaching no chlamydoconidium. The hypha subsequently dissociated yeast-like cells. | |||||
書誌情報 |
Yonago Acta medica en : Yonago Acta medica 巻 41, 号 2, p. 65-72, 発行日 1998-07 |
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出版者 | ||||||
出版者 | Tottori University Faculty of Medicine | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13468049 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00892882 | |||||
権利 | ||||||
権利情報 | Yonago Acta medica 編集委員会 | |||||
情報源 | ||||||
関連名称 | Yonago Acta medica. 1998, 41(2), 65-72 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |