WEKO3
アイテム
Characterization of MORN2 stability and regulatory function in LC3-associated phagocytosis in macrophages
https://repository.lib.tottori-u.ac.jp/records/7110
https://repository.lib.tottori-u.ac.jp/records/7110d791ea89-ea9b-466c-b21a-01b9797d978a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
bo9(6)_bio_051029.pdf (5.5 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
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| 公開日 | 2021-11-25 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Characterization of MORN2 stability and regulatory function in LC3-associated phagocytosis in macrophages | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| キーワード | ||||||||||||
| 主題 | LC3-associated phagocytosis | |||||||||||
| キーワード | ||||||||||||
| 主題 | Membrane trafficking | |||||||||||
| キーワード | ||||||||||||
| 主題 | MORN2 | |||||||||||
| キーワード | ||||||||||||
| 主題 | Non-canonical autophagy | |||||||||||
| キーワード | ||||||||||||
| 主題 | Phagosome maturation | |||||||||||
| キーワード | ||||||||||||
| 主題 | SNARE protein | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題 | LC3-associated phagocytosis | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題 | Membrane trafficking | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題 | MORN2 | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題 | Non-canonical autophagy | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題 | Phagosome maturation | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題 | SNARE protein | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ | journal article | |||||||||||
| 著者 |
森田, 真矢
× 森田, 真矢× 櫻井, 千恵× 堀, 直裕× 初沢, 清隆
WEKO
4750
× Morita, Maya× Kajiye, Mayu× Kubo, Shuichi× Takahashi, Miki× Kinoshita, Daiki |
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| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | Microtubule-associated protein A1/B1-light chain 3 (LC3)-associated phagocytosis (LAP) is a type of non-canonical autophagy that regulates phagosome maturation in macrophages. However, the role and regulatory mechanism of LAP remain largely unknown. Recently, the membrane occupation and recognition nexus repeat-containing-2 (MORN2) was identified as a key component of LAP for the efficient formation of LC3-recruiting phagosomes. To characterize MORN2 and elucidate its function in LAP, we established a MORN2-overexpressing macrophage line. At a steady state, MORN2 was partially cleaved by the ubiquitin-proteasome system. MORN2 overexpression promoted not only LC3-II production but also LAP phagosome (LAPosome) acidification during Escherichia coli uptake. Furthermore, the formation of LAPosomes containing the yeast cell wall component zymosan was enhanced in MORN2-overexpressing cells and depended on reactive oxygen species (ROS). Finally, MORN2-mediated LAP was regulated by plasma membrane-localized soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) such as SNAP-23 and syntaxin 11. Taken together, these findings demonstrate that MORN2, whose expression is downregulated via proteasomal digestion, is a limiting factor for LAP, and that membrane trafficking by SNARE proteins is involved in MORN2-mediated LAP. | |||||||||||
| 書誌情報 |
Biology Open en : Biology Open 巻 9, 号 6, 発行日 2020-06 |
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| 出版者 | ||||||||||||
| 出版者 | The Company of Biologists | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 20466390 | |||||||||||
| DOI | ||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.1242/bio.051029 | |||||||||||
| 権利 | ||||||||||||
| 権利情報 | © 2020. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and repr | |||||||||||
| 情報源 | ||||||||||||
| 関連名称 | Morita Maya, Kajiye Mayu, Sakurai Chiye, et al. Characterization of MORN2 stability and regulatory function in LC3-associated phagocytosis in macrophages. Biology open. 2020. 9(6). doi: 10.1242/bio.051029 | |||||||||||
| 関連サイト | ||||||||||||
| 識別子タイプ | URI | |||||||||||
| 関連識別子 | https://journals.biologists.com/bio/article/9/6/bio051029/222866 | |||||||||||
| 関連名称 | https://journals.biologists.com/bio/article/9/6/bio051029/222866 | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
