| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2021-11-25 |
| タイトル |
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|
タイトル |
Characterization of MORN2 stability and regulatory function in LC3-associated phagocytosis in macrophages |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
LC3-associated phagocytosis |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Membrane trafficking |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
MORN2 |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Non-canonical autophagy |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Phagosome maturation |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
SNARE protein |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
LC3-associated phagocytosis |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Membrane trafficking |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
MORN2 |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Non-canonical autophagy |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
Phagosome maturation |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
SNARE protein |
| 資源タイプ |
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資源タイプ |
journal article |
| 著者 |
森田, 真矢
櫻井, 千恵
堀, 直裕
初沢, 清隆
Morita, Maya
Kajiye, Mayu
Kubo, Shuichi
Takahashi, Miki
Kinoshita, Daiki
|
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University |
| 抄録 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Microtubule-associated protein A1/B1-light chain 3 (LC3)-associated phagocytosis (LAP) is a type of non-canonical autophagy that regulates phagosome maturation in macrophages. However, the role and regulatory mechanism of LAP remain largely unknown. Recently, the membrane occupation and recognition nexus repeat-containing-2 (MORN2) was identified as a key component of LAP for the efficient formation of LC3-recruiting phagosomes. To characterize MORN2 and elucidate its function in LAP, we established a MORN2-overexpressing macrophage line. At a steady state, MORN2 was partially cleaved by the ubiquitin-proteasome system. MORN2 overexpression promoted not only LC3-II production but also LAP phagosome (LAPosome) acidification during Escherichia coli uptake. Furthermore, the formation of LAPosomes containing the yeast cell wall component zymosan was enhanced in MORN2-overexpressing cells and depended on reactive oxygen species (ROS). Finally, MORN2-mediated LAP was regulated by plasma membrane-localized soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) such as SNAP-23 and syntaxin 11. Taken together, these findings demonstrate that MORN2, whose expression is downregulated via proteasomal digestion, is a limiting factor for LAP, and that membrane trafficking by SNARE proteins is involved in MORN2-mediated LAP. |
| 書誌情報 |
Biology Open
en : Biology Open
巻 9,
号 6,
発行日 2020-06
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| 出版者 |
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出版者 |
The Company of Biologists |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
20466390 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1242/bio.051029 |
| 権利 |
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|
権利情報 |
© 2020. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and repr |
| 情報源 |
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関連名称 |
Morita Maya, Kajiye Mayu, Sakurai Chiye, et al. Characterization of MORN2 stability and regulatory function in LC3-associated phagocytosis in macrophages. Biology open. 2020. 9(6). doi: 10.1242/bio.051029 |
| 関連サイト |
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識別子タイプ |
URI |
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関連識別子 |
https://journals.biologists.com/bio/article/9/6/bio051029/222866 |
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関連名称 |
https://journals.biologists.com/bio/article/9/6/bio051029/222866 |
| 著者版フラグ |
|
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出版タイプ |
VoR |
bo9(6)_bio_051029.pdf (5.5 MB)
