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NEAT1 is Required for the Expression of the Liver Cancer Stem Cell Marker CD44
https://repository.lib.tottori-u.ac.jp/records/7230
https://repository.lib.tottori-u.ac.jp/records/7230eba375a9-2f82-4a9f-9838-45082b04ac70
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ijms21_ijms21061927.pdf (6.8 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2021-09-08 | |||||
| タイトル | ||||||
| タイトル | NEAT1 is Required for the Expression of the Liver Cancer Stem Cell Marker CD44 | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | cancer stem cells | |||||
| キーワード | ||||||
| 主題 | NEAT1 | |||||
| キーワード | ||||||
| 主題 | hepatocellular carcinoma | |||||
| キーワード | ||||||
| 主題 | CD44 | |||||
| キーワード | ||||||
| 主題 | spheroid | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | cancer stem cells | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | NEAT1 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | hepatocellular carcinoma | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | CD44 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | spheroid | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
土谷, 博之
× 土谷, 博之× 網崎, 正孝× 本城, 総一郎× 藤原, 義之× 汐田, 剛史× Koyama, Shigemi× Sakaguchi, Hiromi |
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| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Radiology, Department of Pathophysiological and Therapeutic Science, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Molecular and Genetic Medicine, Graduate School of Medicine, Tottori University | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | CD44, a cancer stem cell (CSC) marker, is required for maintaining CSC properties in hepatocellular carcinoma (HCC). Nuclear enriched abundant transcript 1 (NEAT1), a long noncoding RNA (lncRNA), is an oncogenic driver in HCC. In the present study, we investigated the significance of the NEAT1 gene in association with CD44 expression in liver CSCs of human HCC cell lines. The CSC properties were evaluated by spheroid culture, CSC marker expression, and sensitivity to anti-cancer drugs. The expression of both NEAT1 variant 1 (NEAT1v1) and variant 2 (NEAT1v2) as well as CD44 was significantly increased in the spheroid culture, compared with that in monolayer culture. Overexpression of Neat1v1, but not Neat1v2, enhanced the CSC properties, while knockout of the NEAT1 gene suppressed them. CD44 expression was increased by the overexpression of Neat1v1 and abrogated by NEAT1 knockout. The overexpression of NEAT1v1 restored the CSC properties and CD44 expression in NEAT1-knockout cells. NEAT1v1 expression in HCC tissues was correlated with poor prognosis and CD44 expression. These results suggest that NEAT1v1 is required for CD44 expression. To our surprise, NEAT1v1 also restored the CSC properties even in CD44-deficient cells, suggesting that NEAT1v1 maintains the properties of CSCs in a CD44-independent manner. | |||||
| 書誌情報 |
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES en : INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 巻 21, 号 6, 発行日 2020-03 |
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| 出版者 | ||||||
| 出版者 | MDPI | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.3390/ijms21061927 | |||||
| 権利 | ||||||
| 権利情報 | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | |||||
| 情報源 | ||||||
| 関連名称 | Koyama Shigemi, Tsuchiya Hiroyuki, Amisaki Masataka, et al. NEAT1 is Required for the Expression of the Liver Cancer Stem Cell Marker CD44. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2020. 21(6). doi:10.3390/ijms21061927 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.mdpi.com/1422-0067/21/6/1927 | |||||
| 関連名称 | https://www.mdpi.com/1422-0067/21/6/1927 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| EISSN | ||||||
| 値 | 14220067 | |||||
