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L-Ascorbate Biosynthesis Involves Carbon Skeleton Rearrangement in the Nematode Caenorhabditis elegans
https://repository.lib.tottori-u.ac.jp/records/7300
https://repository.lib.tottori-u.ac.jp/records/7300dae9d67e-0fd4-4e57-8243-7126b76ebbc2
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
mtb10(8)_metabo10080334.pdf (2.9 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2022-08-26 | |||||
| タイトル | ||||||
| タイトル | L-Ascorbate Biosynthesis Involves Carbon Skeleton Rearrangement in the Nematode Caenorhabditis elegans | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | L-ascorbate (AsA) | |||||
| キーワード | ||||||
| 主題 | antioxidant | |||||
| キーワード | ||||||
| 主題 | biosynthetic pathway | |||||
| キーワード | ||||||
| 主題 | Caenorhabditis elegans | |||||
| キーワード | ||||||
| 主題 | reactive oxygen species | |||||
| キーワード | ||||||
| 主題 | redox | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | L-ascorbate (AsA) | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | antioxidant | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | biosynthetic pathway | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | Caenorhabditis elegans | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | reactive oxygen species | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | redox | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
藪田, 行哲
× 藪田, 行哲× 美藤, 友博× 石原, 亨× 渡邉, 文雄× Nagata, Ryuta× Aoki, Yuka× Kariya, Ayumi× Wada, Kousuke× Yanagimoto, Ayako× Hara, Hiroka× Okamoto, Naho× Yoshida, Shinichi |
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| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | The United Graduate School of Agricultural Sciences, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Electronic and Organic Material Laboratory, Tottori Institute of Industrial Technology | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Ascorbate (AsA) is required as a cofactor and is widely distributed in plants and animals. Recently, it has been suggested that the nematode Caenorhabditis elegans also synthesizes AsA. However, its biosynthetic pathway is still unknown. To further understand AsA biosynthesis in C. elegans, we analyzed the incorporation of the 13C atom into AsA using gas chromatography-mass spectrometry (GC-MS) in worms fed with D-Glc (1-13C)-labeled Escherichia coli. GC-MS analysis revealed that AsA biosynthesis in C. elegans, similarly to that in mammalian systems, involves carbon skeleton rearrangement. The addition of L-gulono-1,4-lactone, an AsA precursor in the mammalian pathway, significantly increased AsA level in C. elegans, whereas the addition of L-galactono-1,4-lactone, an AsA precursor in the plant and Euglena pathway, did not affect AsA level. The suppression of E03H4.3 (an ortholog of gluconolactonase) or the deficiency of F54D5.12 (an ortholog of L-gulono-1,4-lactone oxidase) significantly decreased AsA level in C. elegans. Although N2- and AsA-deficient F54D5.12 knockout mutant worm (tm6671) morphologies and the ratio of collagen to non-collagen protein did not show any significant differences, the mutant worms exhibited increased malondialdehyde levels and reduced lifespan compared with the N2 worms. In conclusion, our findings indicate that the AsA biosynthetic pathway is similar in C. elegans and mammals. | |||||
| 書誌情報 |
METABOLITES en : METABOLITES 巻 10, 号 8, p. 334, 発行日 2020-08 |
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| 出版者 | ||||||
| 出版者 | MDPI | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.3390/metabo10080334 | |||||
| 権利 | ||||||
| 権利情報 | (C) 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | |||||
| 情報源 | ||||||
| 関連名称 | Yabuta Yukinori, Nagata Ryuta, Aoki Yuka, et al. L-Ascorbate Biosynthesis Involves Carbon Skeleton Rearrangement in the Nematode Caenorhabditis elegans. METABOLITES. 2020. 10(8). doi:10.3390/metabo10080334 | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.3390/metabo10080334 | |||||
| 関連名称 | https://doi.org/10.3390/metabo10080334 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| EISSN | ||||||
| 値 | 22181989 | |||||
