WEKO3
アイテム
Syntaxin 11 regulates the stimulus-dependent transport of Toll-like receptor 4 to the plasma membrane by cooperating with SNAP-23 in macrophages
https://repository.lib.tottori-u.ac.jp/records/7309
https://repository.lib.tottori-u.ac.jp/records/73096aafea7a-b3d7-4577-be8c-c23c9dace66a
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
mbc30(9)_1085.pdf (5.7 MB)
|
|
| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2021-11-25 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Syntaxin 11 regulates the stimulus-dependent transport of Toll-like receptor 4 to the plasma membrane by cooperating with SNAP-23 in macrophages | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ | journal article | |||||||||||
| 著者 |
木下, 大生
× 木下, 大生× 櫻井, 千恵× 堀, 直裕× 初沢, 清隆
WEKO
4750
× Kinoshita, Daiki× Morita, Maya× Tsunematsu, Masashi |
|||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | Syntaxin 11 (stx11) is a soluble N-ethylmaleimide–sensitive factor attachment protein receptor (SNARE) that is selectively expressed in immune cells; however, its precise role in macrophages is unclear. We showed that stx11 knockdown reduces the phagocytosis of Escherichia coli in interferon-γ–activated macrophages. stx11 knockdown decreased Toll-like receptor 4 (TLR4) localization on the plasma membrane without affecting total expression. Plasma membrane–localized TLR4 was primarily endocytosed within 1 h by lipopolysaccharide (LPS) stimulation and gradually relocalized 4 h after removal of LPS. This relocalization was significantly impaired by stx11 knockdown. The lack of TLR4 transport to the plasma membrane is presumably related to TLR4 degradation in acidic endosomal organelles. Additionally, an immunoprecipitation experiment suggested that stx11 interacts with SNAP-23, a plasma membrane–localized SNARE protein, whose depletion also inhibits TLR4 replenishment in LPS-stimulated cells. Using an intramolecular Förster resonance energy transfer (FRET) probe for SNAP-23, we showed that the high FRET efficiency caused by LPS stimulation is reduced by stx11 knockdown. These findings suggest that stx11 regulates the stimulus-dependent transport of TLR4 to the plasma membrane by cooperating with SNAP-23 in macrophages. Our results clarify the regulatory mechanisms underlying intracellular transport of TLR4 and have implications for microbial pathogenesis and immune responses. | |||||||||||
| 書誌情報 |
MOLECULAR BIOLOGY OF THE CELL en : MOLECULAR BIOLOGY OF THE CELL 巻 30, 号 9, p. 1051-1128, 発行日 2019-04-15 |
|||||||||||
| 出版者 | ||||||||||||
| 出版者 | American Society for Cell Biology | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 10591524 | |||||||||||
| DOI | ||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.1091/mbc.E18-10-0653 | |||||||||||
| 権利 | ||||||||||||
| 権利情報 | © 2019 Kinoshita et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative | |||||||||||
| 情報源 | ||||||||||||
| 関連名称 | Kinoshita, Daiki, Sakurai Chiye, Morita Maya et al. Syntaxin 11 regulates the stimulus-dependent transport of Toll-like receptor 4 to the plasma membrane by cooperating with SNAP-23 in macrophages. Molecular biology of the cell, 2019, 30(9), 1085-1097. | |||||||||||
| 関連サイト | ||||||||||||
| 識別子タイプ | URI | |||||||||||
| 関連識別子 | https://www.molbiolcell.org/doi/10.1091/mbc.E18-10-0653 | |||||||||||
| 関連名称 | https://www.molbiolcell.org/doi/10.1091/mbc.E18-10-0653 | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| EISSN | ||||||||||||
| 値 | 19394586 | |||||||||||
