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Novel molecular marker-assisted strategy for production of wheat-Leymus mollis chromosome addition lines
https://repository.lib.tottori-u.ac.jp/records/7371
https://repository.lib.tottori-u.ac.jp/records/73712f16077a-ce8f-44e6-af7b-c6ec505f121d
| 名前 / ファイル | ライセンス | アクション |
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sr8_s41598-018-34545-x.pdf (2.7 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2022-09-08 | |||||
| タイトル | ||||||
| タイトル | Novel molecular marker-assisted strategy for production of wheat-Leymus mollis chromosome addition lines | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
辻本, 壽
× 辻本, 壽× Edet, Offiong U.× Gorafi, Yasir S. A.× Cho, Seong-woo× Kishii, Masahiro |
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| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Arid Land Research Center, Tottori University / United Graduate School of Agricultural Sciences, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Arid Land Research Center, Tottori University / Agricultural Research Corporation (ARC) | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Crop Science and Biotechnology, Chonbuk National University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | International Maize and Wheat Improvement Center (CIMMYT) | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Arid Land Research Center, Tottori University | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Developing wheat–alien chromosome introgression lines to improve bread wheat’s resistance to stresses, such as drought, salinity stress and diseases, requires reliable markers to identify and characterize the alien chromatins. Leymus mollis is a wild relative of bread wheat resistant to salinity and economically important diseases of wheat, but its genome sequence and cytological markers are not available. We devised a molecular marker-assisted strategy for L. mollis chromosome identification and applied it to produce 10 wheat–L. mollis chromosome addition lines. Using 47 L. racemosus genome polymorphic PCR markers and DArTseq genotyping, we distinguished the L. mollis chromosomes and differentiated disomic and monosomic lines by progeny test. DArTseq genotyping generated 14,530 L. mollis SNP markers and the chromosome-specific SNP markers were used to determine the homoeologous groups of L. mollis chromosomes in the addition lines. To validate the marker-based results, genomic in situ hybridization was applied to confirm the presence and cytological status of L. mollis chromosomes in the lines. This study demonstrates that adequate molecular markers allow the production and characterization of wheat–alien addition lines without in situ hybridization, which saves considerable time and effort. | |||||
| 書誌情報 |
SCIENTIFIC REPORTS en : SCIENTIFIC REPORTS 巻 8, p. 16117, 発行日 2018-10-31 |
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| 出版者 | ||||||
| 出版者 | Springer Nature | |||||
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| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 20452322 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1038/s41598-018-34545-x | |||||
| 権利 | ||||||
| 権利情報 | (C) The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the o | |||||
| 情報源 | ||||||
| 関連名称 | Edet Offiong U., Gorafi Yasir S. A., Cho Seong-woo, et al. Novel molecular marker-assisted strategy for production of wheat-Leymus mollis chromosome addition lines. SCIENTIFIC REPORTS. 2018. 8. doi:10.1038/s41598-018-34545-x | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1038/s41598-018-34545-x | |||||
| 関連名称 | https://doi.org/10.1038/s41598-018-34545-x | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
