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A novel method for assessing the renal biopsy specimens using an activatable fluorescent probe
https://repository.lib.tottori-u.ac.jp/records/7373
https://repository.lib.tottori-u.ac.jp/records/7373587722e8-5dcd-4bcc-8877-13d0f490b07d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
sr10_s41598-020-69077-w.pdf (3.8 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2021-03-17 | |||||
| タイトル | ||||||
| タイトル | A novel method for assessing the renal biopsy specimens using an activatable fluorescent probe | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
高田, 知朗
× 高田, 知朗× ヤマダ, ケンタロウ× タニグチ, ソウスケ× 福田, 佐登子× 杉原, 誉明× 磯本, 一× Iyama, Takuji× Yamada, Kentaro× Mae, Yukari× Taniguchi, Sosuke× Ida, Ayami× Ogawa, Masaya× Yamamoto, Marie× Hamada, Shintaro× Kanda, Tsutomu× Urano, Yasuteru |
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| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine / These authors contributed equally: Takuji Iyama and Tomoaki Takata. | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine / These authors contributed equally: Takuji Iyama and Tomoaki Takata. | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Division of Gastroenterology and Nephrology, Tottori University Faculty of Medicine | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Laboratory of Chemical Biology and Molecular Imaging, Graduate School of Medicine, The University of Tokyo | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Gamma-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG) is an activatable fluorescent probe that can be activated by γ-glutamyltranspeptidase (GGT). The expression of GGT in the kidney, which is one of the major organs exhibiting enhanced GGT expression, is exclusively localised to the cortex. Here, we aimed to investigate the feasibility of gGlu-HMRG as a probe for the on-site assessment of renal biopsy specimens. gGlu-HMRG fluorescent probe was applied to the renal proximal tubular epithelial cells and cortical collecting duct cells in vitro, mouse kidneys ex vivo, and human biopsy specimens. In addition, the fluorescence intensities in the cortex and the medulla were comparatively evaluated in the biopsy specimens. The fluorescence signal was rapidly detected in the renal proximal tubular epithelial cells, whereas that in the cortical collecting duct cells was not detected. The fluorescence signal was detected in the mouse kidneys ex vivo without markedly affecting the tissue morphology. In the human biopsy specimens, the fluorescence signal in the cortex was significantly distinct from that in the medulla (p?<?0.05). Thus, this fluorescent probe can be used to distinctly identify the renal cortex in the biopsy specimens. | |||||
| 書誌情報 |
SCIENTIFIC REPORTS en : SCIENTIFIC REPORTS 巻 10, 発行日 2020-07 |
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| 出版者 | ||||||
| 出版者 | NATURE PUBLISHING GROUP | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 20452322 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1038/s41598-020-69077-w | |||||
| 権利 | ||||||
| 権利情報 | (C) The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the o | |||||
| 情報源 | ||||||
| 関連名称 | Iyama Takuji, Takata Tomoaki, Yamada Kentaro, et al. A novel method for assessing the renal biopsy specimens using an activatable fluorescent probe. SCIENTIFIC REPORTS. 2020. 10. doi:10.1038/s41598-020-69077-w | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.nature.com/articles/s41598-020-69077-w | |||||
| 関連名称 | https://www.nature.com/articles/s41598-020-69077-w | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
