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Coenzyme Q10 suppresses apoptosis of mouse pancreatic beta-cell line MIN6
https://repository.lib.tottori-u.ac.jp/records/7161
https://repository.lib.tottori-u.ac.jp/records/7161039e3f17-924a-4308-a6ec-8b53edb3a50b
| 名前 / ファイル | ライセンス | アクション |
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dms10_s13098-018-0351-4.pdf (1.4 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
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| 公開日 | 2021-09-15 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Coenzyme Q10 suppresses apoptosis of mouse pancreatic beta-cell line MIN6 | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| キーワード | ||||||||||||
| 主題 | MIN6 | |||||||||||
| キーワード | ||||||||||||
| 主題 | Coenzyme Q10 | |||||||||||
| キーワード | ||||||||||||
| 主題 | Staurosporine | |||||||||||
| キーワード | ||||||||||||
| 主題 | Apoptosis | |||||||||||
| キーワード | ||||||||||||
| 主題 | Mitochondrial diabetes | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題 | MIN6 | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題 | Coenzyme Q10 | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題 | Staurosporine | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題 | Apoptosis | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題 | Mitochondrial diabetes | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ | journal article | |||||||||||
| 著者 |
角, 啓佑
× 角, 啓佑× 大倉, 毅× 藤岡, 洋平× 加藤, 雅彦× 今村, 武史× 谷口, 晋一
WEKO
3805
× 山本, 一博× Sumi, Keisuke |
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| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Endocrinology and Metabolism, Molecular Medicine and Therapeutics, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Endocrinology and Metabolism, Molecular Medicine and Therapeutics, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Endocrinology and Metabolism, Molecular Medicine and Therapeutics, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Cardiovascular Medicine, Endocrinology and Metabolism, Department of Molecular Medicine and Therapeutics, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Pharmacology, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Department of Regional Medicine, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Cardiovascular Medicine, Endocrinology and Metabolism, Department of Molecular Medicine and Therapeutics, Faculty of Medicine, Tottori University | |||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | Background: In mitochondrial diabetes, apoptosis of β-cells caused by mitochondrial stress plays an important role in impaired insulin secretion. Several studies have reported that coenzyme Q10 (CoQ10) has therapeutic effects on mitochondrial diabetes, but no reports have examined the fundamental effectiveness or mechanism of CoQ10 in mitochondrial diabetes. We previously reported in a Japanese article that CoQ10 has protective effects on pancreatic β-cells against mitochondrial stress using mouse pancreatic β-cell line MIN6 and staurosporine (STS). Here, we report that CoQ10 protects MIN6 cells against apoptosis caused by STS and describe the more detailed apoptotic cascade. Methods: Apoptosis of MIN6 cells was induced by 0.5 μM STS treatment for specific periods with or without 30 μM CoQ10. The apoptosis cascade in MIN6 cells was then investigated using WST-8 assays, annexin-V staining, western blotting, and DNA degradation analysis. Results: Sixteen hours of 0.5 μM STS treatment led to 47% cell viability, but pretreatment with 30 μM CoQ10 resulted in significantly higher viability of 76% (P < 0.01). CoQ10 also prevented translocation of phosphatidylserine from the inner leaflet to the outer leaflet of the cell membrane. CoQ10 prevented cytochrome c release from mitochondria and activation of caspase-3. Conclusion: We concluded that CoQ10 protects pancreatic β-cells through anti-apoptotic effects against STS treatment. | |||||||||||
| 書誌情報 |
DIABETOLOGY & METABOLIC SYNDROME en : DIABETOLOGY & METABOLIC SYNDROME 巻 10, 発行日 2018-06-14 |
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| 出版者 | ||||||||||||
| 出版者 | BioMed Central Ltd. | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 17585996 | |||||||||||
| DOI | ||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.1186/s13098-018-0351-4 | |||||||||||
| 権利 | ||||||||||||
| 権利情報 | © The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creat iveco mmons .org/licen ses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium | |||||||||||
| 情報源 | ||||||||||||
| 関連名称 | Sumi Keisuke, Okura Tsuyoshi, Fujioka Youhei, et al. Coenzyme Q10 suppresses apoptosis of mouse pancreatic beta-cell line MIN6. DIABETOLOGY & METABOLIC SYNDROME. 2018. 10. doi:10.1186/s13098-018-0351-4 | |||||||||||
| 関連サイト | ||||||||||||
| 識別子タイプ | URI | |||||||||||
| 関連識別子 | https://dmsjournal.biomedcentral.com/articles/10.1186/s13098-018-0351-4 | |||||||||||
| 関連名称 | https://dmsjournal.biomedcentral.com/articles/10.1186/s13098-018-0351-4 | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
