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Quantitative analysis of phagosome formation and maturation using an Escherichia coli probe expressing a tandem fluorescent protein
https://repository.lib.tottori-u.ac.jp/records/7242
https://repository.lib.tottori-u.ac.jp/records/7242d960cdbc-ad18-414f-b0c6-7739ebdb7f41
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
jb162(5)_309.pdf (2.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
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| 公開日 | 2021-11-26 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Quantitative analysis of phagosome formation and maturation using an Escherichia coli probe expressing a tandem fluorescent protein | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| キーワード | ||||||||||||
| 主題 | Escherichia coli | |||||||||||
| キーワード | ||||||||||||
| 主題 | macrophage | |||||||||||
| キーワード | ||||||||||||
| 主題 | phagocytosis | |||||||||||
| キーワード | ||||||||||||
| 主題 | phagosome maturation | |||||||||||
| キーワード | ||||||||||||
| 主題 | tandem fluorescent protein | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題 | Escherichia coli | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題 | macrophage | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題 | phagocytosis | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題 | phagosome maturation | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題 | tandem fluorescent protein | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ | journal article | |||||||||||
| 著者 |
櫻井, 千恵
× 櫻井, 千恵× 堀, 直裕× 初沢, 清隆
WEKO
4750
× Morita, Maya× Sawaki, Kazumasa× Kinoshita, Daiki |
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| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||||||||
| 著者所属（英） | ||||||||||||
| 言語 | en | |||||||||||
| 値 | Division of Molecular Biology, School of Life Sciences, Faculty of Medicine, Tottori University | |||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | Phagosome formation and maturation are essential innate immune mechanisms to engulf and digest foreign particles. To analyze these processes quantitatively, we established a specific Escherichia coli probe expressing a tandem fluorescent protein, comprising glutathione S-transferase fused with monomeric Cherry (mCherry) and monomeric Venus (mVenus). We demonstrated that mVenus was more susceptible to bleaching in an acidic environment than mCherry, and that the mVenus:mCherry fluorescence intensity ratio can be used to monitor phagosomal pH changes during maturation. Using this probe, we revealed that synaptosomal-associated protein of 23 kDa, a plasma membrane soluble N-ethylmaleimide-sensitive factor attachment protein receptor protein, actively regulated phagocytosis of E. coli and subsequent phagosome maturation in macrophages. Our results indicated that this probe has the potential to be a powerful tool in understanding the molecular mechanisms of phagosome formation and maturation. | |||||||||||
| 書誌情報 |
JOURNAL OF BIOCHEMISTRY en : JOURNAL OF BIOCHEMISTRY 巻 162, 号 5, p. 309-316, 発行日 2017-11 |
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| 出版者 | ||||||||||||
| 出版者 | Oxford University Press | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 0021924X | |||||||||||
| DOI | ||||||||||||
| 関連タイプ | isVersionOf | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | 10.1093/jb/mvx034 | |||||||||||
| 権利 | ||||||||||||
| 権利情報 | This is a pre-copyedited, author-produced version of an article accepted for publication in Journal of biochemistry following peer review. The version of record Morita Maya, Sawaki Kazumasa, Kinoshita Daiki, et al. Quantitative analysis of phagosome forma | |||||||||||
| 情報源 | ||||||||||||
| 関連名称 | Morita Maya, Sawaki Kazumasa, Kinoshita Daiki, et al. Quantitative analysis of phagosome formation and maturation using an Escherichia coli probe expressing a tandem fluorescent protein. JOURNAL OF BIOCHEMISTRY. 2017. 162(5). 309-316. doi:10.1093/jb/mvx03 | |||||||||||
| 関連サイト | ||||||||||||
| 識別子タイプ | URI | |||||||||||
| 関連識別子 | https://academic.oup.com/jb/article/162/5/309/3858071 | |||||||||||
| 関連名称 | https://academic.oup.com/jb/article/162/5/309/3858071 | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | AM | |||||||||||
| EISSN | ||||||||||||
| 値 | 17562651 | |||||||||||
