| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2022-07-06 |
| タイトル |
|
|
タイトル |
Horseradish Peroxidase-Decorated Artificial Viral Capsid Constructed from β-Annulus Peptide via Interaction between His-Tag and Ni-NTA |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
artificial viral capsid |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
self-assembly |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
β-annulus peptide |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
horseradish peroxidase |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
nanocapsule |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
surface decoration |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
artificial viral capsid |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
self-assembly |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
β-annulus peptide |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
horseradish peroxidase |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
nanocapsule |
| キーワード |
|
|
言語 |
en |
|
主題Scheme |
Other |
|
主題 |
surface decoration |
| 資源タイプ |
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|
資源タイプ |
journal article |
| 著者 |
松浦, 和則
稲葉, 央
Shiomi, Yuriko
Mizuta, Toshihumi
|
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University / Centre for Research on Green Sustainable Chemistry, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Technical Department, Tottori University |
| 著者所属（英） |
|
|
言語 |
en |
|
値 |
Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University / Centre for Research on Green Sustainable Chemistry, Tottori University |
| 抄録 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Artificial construction of spherical protein assemblies has attracted considerable attention due to its potential use in nanocontainers, nanocarriers, and nanoreactors. In this work, we demonstrate a novel strategy to construct peptide nanocapsules (artificial viral capsids) decorated with enzymes via interactions between His-tag and Ni-NTA. A β-annulus peptide derived from the tomato bushy stunt virus was modified with Ni-NTA at the C-terminus, which is directed toward the exterior surface of the artificial viral capsid. The β-annulus peptide bearing Ni-NTA at the C-terminus self-assembled into capsids of about 50 nm in diameter. The Ni-NTA-displayed capsids were complexed with recombinant horseradish peroxidase (HRP) with a C-terminal His-tag which was expressed in Escherichia coli. The β-annulus peptide-HRP complex formed spherical assemblies whose sizes were 30–90 nm, with the ζ-potential revealing that the HRP was decorated on the outer surface of the capsid. |
| 書誌情報 |
PROCESSES
en : PROCESSES
巻 8,
号 11,
発行日 2020-11
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| 出版者 |
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出版者 |
MDPI |
| DOI |
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関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
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関連識別子 |
10.3390/pr8111455 |
| 権利 |
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|
権利情報 |
(C) 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
| 情報源 |
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関連名称 |
Matsuura Kazunori, Shiomi Yuriko, Mizuta Toshihumi, et al. Horseradish Peroxidase-Decorated Artificial Viral Capsid Constructed from β-Annulus Peptide via Interaction between His-Tag and Ni-NTA. PROCESSES. 2020. 8(11). doi:10.3390/pr8111455 |
| 関連サイト |
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|
識別子タイプ |
URI |
|
|
関連識別子 |
https://www.mdpi.com/2227-9717/8/11/1455 |
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|
関連名称 |
https://www.mdpi.com/2227-9717/8/11/1455 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
| EISSN |
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|
値 |
22279717 |
prc8(11)_pr8111455.pdf (3.4 MB)
