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Horseradish Peroxidase-Decorated Artificial Viral Capsid Constructed from β-Annulus Peptide via Interaction between His-Tag and Ni-NTA
https://repository.lib.tottori-u.ac.jp/records/7346
https://repository.lib.tottori-u.ac.jp/records/7346d8f5d7f8-54d5-4f6b-82c4-c7b1d88dd948
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
prc8(11)_pr8111455.pdf (3.4 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2022-07-06 | |||||
| タイトル | ||||||
| タイトル | Horseradish Peroxidase-Decorated Artificial Viral Capsid Constructed from β-Annulus Peptide via Interaction between His-Tag and Ni-NTA | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題 | artificial viral capsid | |||||
| キーワード | ||||||
| 主題 | self-assembly | |||||
| キーワード | ||||||
| 主題 | β-annulus peptide | |||||
| キーワード | ||||||
| 主題 | horseradish peroxidase | |||||
| キーワード | ||||||
| 主題 | nanocapsule | |||||
| キーワード | ||||||
| 主題 | surface decoration | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | artificial viral capsid | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | self-assembly | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | β-annulus peptide | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | horseradish peroxidase | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | nanocapsule | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題 | surface decoration | |||||
| 資源タイプ | ||||||
| 資源タイプ | journal article | |||||
| 著者 |
松浦, 和則
× 松浦, 和則× 稲葉, 央× Shiomi, Yuriko× Mizuta, Toshihumi |
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| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University / Centre for Research on Green Sustainable Chemistry, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Technical Department, Tottori University | |||||
| 著者所属（英） | ||||||
| 言語 | en | |||||
| 値 | Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University / Centre for Research on Green Sustainable Chemistry, Tottori University | |||||
| 抄録 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Artificial construction of spherical protein assemblies has attracted considerable attention due to its potential use in nanocontainers, nanocarriers, and nanoreactors. In this work, we demonstrate a novel strategy to construct peptide nanocapsules (artificial viral capsids) decorated with enzymes via interactions between His-tag and Ni-NTA. A β-annulus peptide derived from the tomato bushy stunt virus was modified with Ni-NTA at the C-terminus, which is directed toward the exterior surface of the artificial viral capsid. The β-annulus peptide bearing Ni-NTA at the C-terminus self-assembled into capsids of about 50 nm in diameter. The Ni-NTA-displayed capsids were complexed with recombinant horseradish peroxidase (HRP) with a C-terminal His-tag which was expressed in Escherichia coli. The β-annulus peptide-HRP complex formed spherical assemblies whose sizes were 30–90 nm, with the ζ-potential revealing that the HRP was decorated on the outer surface of the capsid. | |||||
| 書誌情報 |
PROCESSES en : PROCESSES 巻 8, 号 11, 発行日 2020-11 |
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| 出版者 | ||||||
| 出版者 | MDPI | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.3390/pr8111455 | |||||
| 権利 | ||||||
| 権利情報 | (C) 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | |||||
| 情報源 | ||||||
| 関連名称 | Matsuura Kazunori, Shiomi Yuriko, Mizuta Toshihumi, et al. Horseradish Peroxidase-Decorated Artificial Viral Capsid Constructed from β-Annulus Peptide via Interaction between His-Tag and Ni-NTA. PROCESSES. 2020. 8(11). doi:10.3390/pr8111455 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.mdpi.com/2227-9717/8/11/1455 | |||||
| 関連名称 | https://www.mdpi.com/2227-9717/8/11/1455 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| EISSN | ||||||
| 値 | 22279717 | |||||
